Basic Information
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mRNA expression analysis
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Species-specific CFD gene expression analysis in wild-type and humanized B-hCFD mice by RT-PCR. Murine Cfd mRNA was detected in heart tissue isolated from wild-type C57BL/6 (+/+) mice, while human CFD mRNA was exclusively detected in homozygous B-hCFD (H/H) mice.
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Protein expression analysis
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Species-specific CFD protein expression analysis in wild-type and humanized B-hCFD mice. Fat tissue was isolated from wild-type C57BL/6 (+/+) and homozygous B-hCFD (H/H) mice and analyzed by western blot using species-specific anti-FAD antibodies. Human monocytic leukemia cells (THP-1) were used as positive control. Murine CFD protein was detected in wild-type mice, while human CFD protein was exclusively detected in THP-1 cells and B-hCFD mice.
Species-specific CFD protein expression analysis in wild-type and humanized B-hCFD mice. Serum was collected from wild-type C57BL/6 (+/+) and homozygous B-hCFD (H/H) mice and analyzed using species-specific ELISA kits. Murine CFD protein was detected in wild-type mice, while human CFD protein was exclusively detected in B-hCFD mice.
