Basic Information

Strain Name
C57BL/6-Cfdtm1(CFD)/Bcgen
Common Name
B-hCFD mice
Background
C57BL/6
Catalog number
111005
Related Genes
ADIPSIN, ADN, DF, PFD
NCBI Gene ID

mRNA expression analysis

Species-specific CFD gene expression analysis in wild-type and humanized B-hCFD mice by RT-PCR. Murine Cfd mRNA was detected in heart tissue isolated from wild-type C57BL/6 (+/+) mice, while human CFD mRNA was exclusively detected in homozygous B-hCFD (H/H) mice.

Protein expression analysis

Species-specific CFD protein expression analysis in wild-type and humanized B-hCFD mice. Fat tissue was isolated from wild-type C57BL/6 (+/+) and homozygous B-hCFD (H/H) mice and analyzed by western blot using species-specific anti-FAD antibodies. Human monocytic leukemia cells (THP-1) were used as positive control. Murine CFD protein was detected in wild-type mice, while human CFD protein was exclusively detected in THP-1 cells and B-hCFD mice.

 

Species-specific CFD protein expression analysis in wild-type and humanized B-hCFD mice. Serum was collected from wild-type C57BL/6 (+/+) and homozygous B-hCFD (H/H) mice and analyzed using species-specific ELISA kits. Murine CFD protein was detected in wild-type mice, while human CFD protein was exclusively detected in B-hCFD mice.