Basic Information

Strain Name
C57BL/6-Cxcl1tm1(CXCL1)/Bcgen
Common Name
B-hIL18 mice
Background
C57BL/6N
Catalog number
111395
Aliases
CXCL1 (FSP, GRO1, GROa, MGSA; NAP-3, SCYB1, MGSA-a)
NCBI Gene ID

Gene targeting strategy

Gene targeting strategy for B-hCXCL1 mice. 

The exons 1-4 of mouse Cxcl1 gene that encode the full-length protein were replaced by human CXCL1 exons 1-4 in B-hCXCL1 mice.

Protein expression analysis

Species-specific CXCL1 protein expression analysis in humanized B-hCXCL1 mice. Following LPS (20µg/200µl) stimulation in vivo, serum was collected from wild-type C57BL/6 (+/+) and homozygous B-hCXCL1 (H/H) mice and analyzed by ELISA using species-specific CXCL1 kits. Murine CXCL1 protein was detected in wild-type mice, while human CXCL1 protein was detected in B-hCXCL1 mice.

Summary

Protein expression analysis:

Human CXCL1 was detectable in serum of homozygous B-hCXCL1 mice but not in wild-type mice, and mouse CXCL1 was detectable only in wild-type mice.

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