CXCL1 (FSP, GRO1, GROa, MGSA; NAP-3, SCYB1, MGSA-a)
NCBI Gene ID
Gene targeting strategy
Gene targeting strategy for B-hCXCL1 mice. Exons 1-4 of mouse Cxcl1 gene that encode the full-length protein were replaced by human CXCL1 exons 1-4 in B-hCXCL1 mice.
Protein expression analysis
Species-specific CXCL1 protein expression analysis in humanized B-hCXCL1 mice. Following LPS (20µg/200µl) stimulation in vivo, serum was collected from wild-type C57BL/6 (+/+) and homozygous B-hCXCL1 (H/H) mice and analyzed by ELISA using species-specific CXCL1 kits. Murine CXCL1 protein was detected in wild-type mice, while human CXCL1 protein was detected in B-hCXCL1 mice.