Basic Information

Strain Name
C57BL/6-Cxcl13tm1(CXCL13)/Bcgen
Common Name
B-hCXCL13 mice
Catalog Number
111136
Background
C57BL/6
Related Gene
Chemokine (C-X-C motif) ligand 13 (CXCL13), B lymphocyte chemoattractant (BLC) or B cell-attracting chemokine 1 (BCA-1)
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hCXCL13 mice. The exons 1~4 of mouse Cxcl13 gene that encode the full-length protein were replaced by human CXCL13 exons 1~4 in B-hCXCL13 mice.

Details

mRNA expression analysis

Strain specific analysis of CXCL13 gene expression in WT and homozygous B-hCXCL13 mice by RT-PCR. Mouse Cxcl13 mRNA was detectable in splenocytes of wild-type (+/+) mice. Human CXCL13 mRNA was detectable only in H/H, but not in +/+ mice.

Protein expression analysis

Strain specific CXCL13 expression analysis in homozygous B-hCXCL13 mice by ELISA.
Serum were collected from WT and homozygous B-hCXCL13 (H/H) mice, and analyzed by ELISA with species-specific CXCL13 ELISA kit. Mouse CXCL13 was detectable in WT mice. Human CXCL13 was exclusively detectable in homozygous B-hCXCL13 but not WT mice.