Basic Information
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Targeting strategy
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Gene targeting strategy for B-hCXCL16 mice.
The exons 2~5 of mouse Cxcl16 gene were replaced by human CXCL16 exons 2~5 in B-hCXCL16 mice.
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mRNA expression analysis
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Strain specific analysis of CXCL16 mRNA expression in wild-type C57BL/6 mice and B-hCXCL16 mice by RT-PCR. Lung RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hCXCL16 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human CXCL16 primers. Mouse Cxcl16 mRNA was detected in wild-type C57BL/6 mice. Human CXCL16 mRNA was detected in homozygous B-hCXCL16 mice but not in wild-type mice.
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Protein expression analysis
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Strain specific CXCL16 expression analysis in homozygous B-hCXCL16 mice by ELISA. Serum was collected from wild-type mice (+/+) and homozygous B-hCXCL16 mice (H/H), and analyzed by ELISA. Human CXCL16 was detectable in homozygous B-hCXCL16 mice.
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Summary
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mRNA expression analysis:
Mouse Cxcl16 mRNA was detected in lung of wild-type C57BL/6 mice. Human CXCL16 mRNA was detected in lung of B-hCXCL16 mice.
Protein expression analysis:
Human CXCL16 was detectable in homozygous B-hCXCL16 mice.
