FGF21, fibroblast growth factor 21
NCBI Gene ID
Gene targeting strategy
Gene targeting strategy for B-hFGF21 mice. Exons 1-3 of mouse FGF21 gene that encode the full-length protein were replaced by human FGF21 exons 2-4 in B-hFGF21 mice.
Protein expression analysis
Species-specific FGF21 protein expression analysis in wild-type and humanized B-hFGF21 mice. Serum was collected from wild-type C57BL/6N (+/+) and heterozygous B-hFGF21 (H/+) mice and analyzed by ELISA using FGF21 kits. Mouse FGF21 was detected in wild-type and heterozygous B-hFGF21 mice, while human FGF21 was detected in wild-type and B-hFGF21 mice due to the cross-reactive antibody. Values are expressed as mean ± SEM.