Basic Information
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Targeting Strategy
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Gene targeting strategy for B-hFGFR3 mice.
Exons 3-9 of mouse Fgfr3 (which encode the extracellular domain) were replaced by human FGFR3 exons 3-9 in B-hFGFR3 mice.
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FGFR3 mRNA Expression
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Strain specific analysis of FGFR3 gene expression in wild-type mice and B-hFGFR3 mice by RT-PCR. Mouse Fgfr3 mRNA was detectable in lung of wild-type mice (+/+). Human FGFR3 mRNA was detectable in homozygous B-hFGFR3 but not in wild-type mice.
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FGFR3 Protein Expression
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Strain specific FGFR3 expression analysis in homozygous B-hFGFR3 mice by western blot. Brain tissue was collected from wild-type C57BL/6 mice (+/+) and homozygous B-hFGFR3 mice (H/H), and analyzed by western blot with anti-FGFR3 antibody. Human FGFR3 was detectable in wild type mice and homozygous B-hFGFR3 mice, as the antibody is cross-reactive with human and mouse FGFR3 .
