Basic Information

Strain Name
C57BL/6N-Fgfr3tm1(FGFR3)/Bcgen
Common Name
B-hFGFR3 mice
Background
C57BL/6N
Catalog Number
112158
Alias
FGFR3 (ACH; CEK2; JTK4; CD333; HSFGFR3EX)
NCBI Gene ID

Targeting Strategy

Gene targeting strategy for B-hFGFR3 mice.

Exons 3-9 of mouse Fgfr3 (which encode the extracellular domain) were replaced by human FGFR3 exons 3-9 in B-hFGFR3 mice.

FGFR3 mRNA Expression

 

Strain specific analysis of FGFR3 gene expression in wild-type mice and B-hFGFR3 mice by RT-PCR. Mouse Fgfr3 mRNA was detectable in lung of wild-type mice (+/+). Human FGFR3 mRNA was detectable in homozygous B-hFGFR3 but not in wild-type mice.

 

FGFR3 Protein Expression

Strain specific FGFR3 expression analysis in homozygous B-hFGFR3 mice by western blot. Brain tissue was collected from wild-type C57BL/6 mice (+/+) and homozygous B-hFGFR3 mice (H/H), and analyzed by western blot with anti-FGFR3 antibody. Human FGFR3 was detectable in wild type mice and homozygous B-hFGFR3 mice, as the antibody is cross-reactive with human and mouse FGFR3 .