Basic Information
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mRNA expression analysis
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Strain specific analysis of GFRAL gene expression in wild-type mice and B-hGFRAL mice by RT-PCR. Mouse Gfral mRNA was detectable in brain of wild-type mice (+/+). Human GFRAL mRNA was detectable only in brain of homozygous B-hGFRAL mice but not in wild-type mice.
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Protein expression analysis
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Strain specific analysis of GFRAL expression in C57BL/6(+/+) and homozygous B-hGFRAL(H/H) mice by western blot. Ovary and stomach were collected from C57BL/6(+/+) and homozygous B-hGFRAL(H/H) mice. GFRAL was detectable in C57BL/6(+/+) and B-hGFRAL (H/H) mice due to the cross-reactivity of antibodies.
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Summary
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mRNA expression
Human GFRAL mRNA was detectable only in brain of homozygous B-hGFRAL mice but not in wild-type mice.
Protein expression
GFRAL was detectable in C57BL/6(+/+) and B-hGFRAL (H/H) mice due to the cross-reactivity of antibodies.