Basic Information

Strain Name
C57BL/6-Grptm1(GRP)/Bcgen
Common Name
B-hGRP mice
Background
C57BL/6
Catalog number
111902
Aliases
GRP (BN, GRP-10, proGRP, preproGRP)
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hGRP mice. 

The exons 1-3 of mouse Grp gene that encode the full-length protein were replaced by human GRP exons 1-3 in B-hGRP mice. 

mRNA and Protein expression analysis

Strain specific analysis of GRP gene expression in wild-type mice and B-hGRP mice by RT-PCR. Mouse Grp mRNA was detectable in brain of wild-type mice (+/+). Human GRP mRNA was detectable only in homozygous B-hGRP but not in wild-type mice.

Strain specific GRP expression analysis in homozygous B-hGRP mice by western blot. Brain tissue was collected from wild-type C57BL/6 mice (+/+) and homozygous B-hGRP mice (H/H), and analyzed by western blot with anti-GRP antibody. GRP was detectable in wild type mice and homozygous B-hGRP mice, as the antibody is crossly reactive with GRP in human and mice.

Summary

  • mRNA expression analysis:

Mouse Grp mRNA was detectable in brain of wild-type (+/+). Human GRP mRNA was detectable in B-hGRP mice (H/H) but not in wild-type mice.

  • Protein expression analysis:

Human GRP was exclusively detectable in homozygous B-hGRP mice.

Back to top