Basic Information
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Targeting strategy
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Gene targeting strategy for B-hIFNAR2 mice. The chimeric CDS consists of human IFNAR2 extracellular domain and mouse Ifnar2 intracellular region was inserted into mouse ifnar2 gene locus in B-hIFNAR2 mice. Mouse Ifnar2 transcript has been disrupted.
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mRNA expression analysis
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Species-specific IFNAR2 gene expression analysis in wild-type and humanized B-hIFNAR2 mice by RT-PCR. Mouse Ifnar2 mRNA was detected in splenocytes isolated from wild-type C57BL/6 (+/+) and heterozygous B-hIFNAR2 (H/+) mice, while human IFNAR2 mRNA was detected in B-hIFNAR2 mice.
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Protein expression analysis
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Human IFNAR2 protein expression analysis in wild-type and humanized B-hIFNAR2 mice. Splenocytes were isolated from wild-type C57BL/6N (+/+) and heterozygous B-hIFNAR2 (H/+) mice and analyzed by flow cytometry using an anti-human IFNAR2 antibody. Human IFNAR2 protein was detected in B cells, T cells, NK cells, DCs, monocytes and macrophages from B-hIFNAR2 mice. According to the results, the antibody also has some un-specific binding with mouse IFNAR2, but the percentage of positive cells was lower in wild-type C57BL/6N mice than heterozygous B-hIFNAR2 mice.
