Basic Information

Strain Name
C57BL/6N-Ifnar2tm1(IFNAR2)/Bcgen
Common Name
B-hIFNAR2 mice
Background
C57BL/6N
Catalog Number
112400
Related Gene
IFN-R; IMD45; IFNABR; IFNARB; IFN-alpha-REC
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hIFNAR2 mice. The chimeric CDS consists of human IFNAR2 extracellular domain and mouse Ifnar2 intracellular region was inserted into mouse ifnar2 gene locus in B-hIFNAR2 mice. Mouse Ifnar2 transcript has been disrupted.

mRNA expression analysis

Species-specific IFNAR2 gene expression analysis in wild-type and humanized B-hIFNAR2 mice by RT-PCR. Mouse Ifnar2 mRNA was detected in splenocytes isolated from wild-type C57BL/6 (+/+) and heterozygous B-hIFNAR2 (H/+) mice, while human IFNAR2 mRNA was detected in B-hIFNAR2 mice.

Protein expression analysis

Human IFNAR2 protein expression analysis in wild-type and humanized B-hIFNAR2 mice. Splenocytes were isolated from wild-type C57BL/6N (+/+) and heterozygous B-hIFNAR2 (H/+) mice and analyzed by flow cytometry using an anti-human IFNAR2 antibody. Human IFNAR2 protein was detected in B cells, T cells, NK cells, DCs, monocytes and macrophages from B-hIFNAR2 mice. According to the results, the antibody also has some un-specific binding with mouse IFNAR2, but the percentage of positive cells was lower in wild-type C57BL/6N mice than heterozygous B-hIFNAR2 mice.

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