Basic Information
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Targeting strategy
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Gene targeting strategy for B-hIFNGR1/hIFNG mice. The full coding sequence of mouse Ifng gene was replaced by counterpart of human IFNG gene in B-hIFNGR1/hIFNG mice. The sequence that encoding the extracellular region of mouse Ifngr1 gene was replaced by counterpart human IFNGR1 gene in B-hIFNGR1/hIFNG mice.
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mRNA expression analysis
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IFNG and IFNGR1 expression analysis in B-hIFNGR1/hIFNG mice by RT-PCR. Human IFNG and IFNGR1 mRNA were detectable in splenocytes of the homozygous B-hIFNGR1/hIFNG mice (H/H,H/H) but not in wild-type mice (+/+).
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Protein expression analysis
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IFNG and IFNGR1 expression analysis in B-hIFNGR1/IFNG mice. (A) IFNG expression analysis. Serum was collected from wild-type mice (+/+) and homozygous B-hIFNGR1/hIFNG mice (H/H,H/H) after anti-CD3ε stimulated for 2 hours, and analyzed by ELISA with species-specific IFNG ELISA kit. Mouse IFNG was exclusively detectable in wild-type mice and human IFNG was exclusively detectable in homozygous B-hIFNGR1/hIFNG mice. (B) IFNGR1 expression analysis. Peritoneal derived macrophage cells were collected from wild-type and B-hIFNGR1/hIFNG mice, and analyzed by flow cytometry with anti-IFNGR1 antibody. Human IFNGR1 was detectable in B-hIFNGR1/hIFNG mice but not in wild-type mice.