Basic Information

Strain Name
C57BL/6-Ifngr1tm1(IFNGR1)Ifngtm1(IFNG)/Bcgen
Common Name
B-hIFNGR1/hIFNG mice
Background
C57BL/6N
Catalog Number
120819
Aliases
IFNG: IFG, IFI, IMD69,IFN-γ IFNGR1: CD119, IFNGR, IMD27A, IMD27B
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hIFNGR1/hIFNG mice. The full coding sequence of mouse Ifng gene was replaced by counterpart of human IFNG gene in B-hIFNGR1/hIFNG mice. The sequence that encoding the extracellular region of mouse Ifngr1 gene was replaced by counterpart human IFNGR1 gene in B-hIFNGR1/hIFNG mice.

mRNA expression analysis

IFNG and IFNGR1 expression analysis in B-hIFNGR1/hIFNG mice by RT-PCR. Human IFNG and IFNGR1 mRNA were detectable in splenocytes of the homozygous B-hIFNGR1/hIFNG mice (H/H,H/H) but not in wild-type mice (+/+).

Protein expression analysis

IFNG and IFNGR1 expression analysis in B-hIFNGR1/IFNG mice. (A) IFNG expression analysis. Serum was collected from wild-type mice (+/+) and homozygous B-hIFNGR1/hIFNG mice (H/H,H/H) after anti-CD3ε stimulated for 2 hours, and analyzed by ELISA with species-specific IFNG ELISA kit. Mouse IFNG was exclusively detectable in wild-type mice and human IFNG was exclusively detectable in homozygous B-hIFNGR1/hIFNG mice. (B) IFNGR1 expression analysis. Peritoneal derived macrophage cells were collected from wild-type and B-hIFNGR1/hIFNG mice, and analyzed by flow cytometry with anti-IFNGR1 antibody. Human IFNGR1 was detectable in B-hIFNGR1/hIFNG mice but not in wild-type mice.

Summary

mRNA expression analysis:

Human IFNG and IFNGR1 mRNA were detectable in splenocytes of the homozygous B-hIFNGR1/hIFNG mice.

Protein expression analysis:

Human IFNG protein was exclusively detectable in serum of homozygous B-hIFNGR1/hIFNG mice.

Human IFNGR1 protein was exclusively detectable in peritoneal derived macrophage cells of homozygous B-hIFNGR1/hIFNG mice.