mRNA and protein expression analysis
IFNGR1 expression analysis in wild-type and humanized B-hIFNGR1 mice. (A) RT-PCR analysis of IFNGR1. Human IFNGR1 mRNA was detected in splenocytes isolated from homozygous B-hIFNGR1 (H/H) mice, while murine Ifngr1 mRNA was only detected in wild-type mice (+/+). (B) Peritoneal-derived macrophages were isolated from wild-type (+/+) and homozygous B-hIFNGR1 (H/H) mice, and analyzed by flow cytometry using species-specific anti-IFNGR1 antibodies. Human IFNGR1 protein was detected in B-hIFNGR1 mice, while murine IFNGR1 protein was detected in wild-type mice.