Basic Information

Strain name
C57BL/6Igf2rtm1(IGF2R)Bcgen/Bcgen
Common Name
B-hIGF2R mice
Stock Number
110986
Background
C57BL/6
Aliases
IGF2R(CD222, CI-M6PR, CIMPR, M6P-R, M6P/IGF2R, MPR 300, MPR1, MPR300, MPRI)
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hIGF2R mice. The extracellular, transmembrane and intracellular region of human IGF2R CDS were inserted into the mouse exon3.

mRNA and protein expression analysis

Strain specific analysis of IGF2R gene expression in wild-type mice and B-hIGF2R mice by RT-PCR. Mouse Igf2r mRNA was detectable in splenocytes of wild-type mice (+/+). Human IGF2R mRNA was detectable only in homozygous B-hIGF2R mice(H/H) but not in wild-type mice.

Strain specific IGF2R expression analysis in homozygous B-hIGF2R mice by western blot. Heart tissue, kidney tissue and spleen tissue were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hIGF2R mice (H/H), and analyzed by western blot with anti-hIGF2R antibody. Human IGF2R was detectable in wild-type mice and homozygous B-hIGF2R mice, as the antibody is crossly reactive with IGF2R in human and mice.

Summary

mRNA and Protein expression analysis:

Human IGF2R mRNA was detectable in B-hIGF2R mice, and we proved that the protein could be expressed normally by western blot analysis.

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