Basic Information

Strain Name
C57BL/6-Il10tm1(IL10)/Bcgen
Common Name
B-hIL10 mice
Background
C57BL/6
Catalog number
110060
Related Genes
Il10 (interleukin 10)
NCBI Gene ID

Details

1.Generation of gene editing mouse model and expression analysis

mRNA expression analysis

 

Strain specific analysis of IL10 gene expression in WT and hIL10 mice by RT-PCR. Mouse Il10 mRNA was detectable in splenocytes of wild-type (+/+) mice, Human IL10 mRNA was detectable only in homozygous (H/H) mice but not WT mice.

Protein expression analysis

Serum from both wild type (WT) C57BL/6 and homozygous B-hIL10 mice were analyzed by ELISA.

When treated with LPS, mouse IL10 was only detectable in WT C57BL/6 mice, while human IL10 was only detectable in homozygous B-hIL10 mice. n=3.

 

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