B-hIL12A/hIL12B/hIL12RB1/hIL12RB2 mice

Basic Information

Strain Name
C57BL/6-Il12atm1(IL12A)Il12btm1(IL12B)Il12rb1tm1(IL12RB1) Il12rb2tm1(IL12RB2)/Bcgen
Common name
B-hIL12A/hIL12B/hIL12RB1/hIL12RB2 mice
Background
C57BL/6
Catalog number
140575
Related genes
IL12A also known as P35, CLMF, NFSK, NKSF1, IL-12A IL12B also known as CLMF, NKSF, CLMF2, IMD28, IMD29, NKSF2, IL-12B IL12RB1 also known as CD212, IMD30, IL12RB, IL-12R-BETA1

Targeting strategy

 

  • The exons 1-7 of mouse Il12a gene that encode the full length coding sequence were replaced by human IL12A exons 1-7 in B-hIL12A/hIL12B/hIL12RB1/hIL12RB2 mice.

 

  • The exons 2-8 of mouse Il12b gene that encode the full length coding sequence and 3’UTR were replaced by human IL12B exons 2-8 in B-hIL12A/hIL12B/hIL12RB1/hIL12RB2 mice.

 

  • The extracellular and transmembrane region coding sequences of human IL12RB1 gene plus the mouse Il12rb1 cytoplasmic coding sequences were inserted into the exon1 of B-hIL12A/hIL12B/hIL12RB1/hIL12RB2 mice.

 

  • The extracellular region coding sequences of human IL12RB2 gene plus the mouse Il12rb2 transmembrane and cytoplasmic coding sequences were inserted into the exon2 of B-hIL12A/hIL12B/hIL12RB1/hIL12RB2 mice.

Protein expression analysis

Strain specific IL12p70 (IL-12A (p35) and IL-12B (p40) active heterodimer referred to as ‘p70’) expression analysis in homozygous B-hIL12A/hIL12B/hIL12RB1/hIL12RB2 mice by ELISA.

Serum was collected in wild-type (+/+) and homozygous B-hIL12A/hIL12B/hIL12RB1/hIL12RB2 mice (H/H) stimulated with anti-mCD3ε and anti-mCD28 in vivo (n=3), and analyzed by ELISA with species-specific IL12 ELISA kit. Mouse IL12p70 was detectable in wild-type mice. Human IL12 p70 was exclusively detectable in homozygous B-hIL12A/hIL12B/hIL12RB1/hIL12RB2 mice but not in wild-type mice. ND: Not detectable.

Poster

AACR 2022: B-hIL12A/hIL12B/hIL12RB1/hIL12RB2: A Novel Animal Model for Generation of IL12 Therapies