Basic Information
Strain Name
C57BL/6-Il3m1(IL13)/Bcgen
Stock Number
110058
Common Name
B-hIL13 mice
Source/Investigator
Bcgen (Beijing Biocytogen Co., Ltd)
Related Genes
Il13(interleukin 13)
Species
C57BL/6J
Appearance
Black
Genotypes
Homozygous
NCBI Gene ID
-
mRNA expression analysis
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Strain specific analysis of IL13 gene expression in WT and B-hIL13 mice by RT-PCR. Mouse Il13 mRNA was detectable in thymocytes of wild-type (+/+) but not in homozygous B-hIL13 mice (H/H) mice. Human IL13 mRNA was detectable only in homozygous B-hIL13 mice but not in wild-type mice.
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Protein expression analysis
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Strain specific IL13 expression analysis in homozygous B-hIL13 mice by ELISA. CD4+ T cells were collected from WT and homozygous B-hIL13 (H/H) mice, and then induction of CD4 + T-cells differentiation into Th2 cells, supernatant from Th2 cells culture was analyzed by ELISA with species-specific IL13 ELISA kit. Mouse Il13 was detectable in WT mice. Human IL13 was exclusively detectable in homozygous B-hIL13 mice but not WT mice.
