Basic Information
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Gene Targeting Strategy
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Gene targeting strategy for B-hIL17RA mice. The exons 2-9 of mouse Il17ra gene that encode the extracellular domain were replaced by human IL17RA exons 2-9 in B-hIL17RA mice.
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Immune Cell Analysis
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Strain specific IL17RA expression analysis in heterozygous B-hIL17RA mice by flow cytometry. Bone marrow was collected from WT and heterozygous B-hIL17RA (H/+) mice, and analyzed by flow cytometry with species-specific anti-IL17RA antibody. Mouse IL17RA was detectable in WT and heterozygous B-hIL17RA mice. Human IL17RA was exclusively detectable in heterozygous B-hIL17RA but not WT mice.
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Summary
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Protein expression analysis:
Human IL17RA was exclusively detectable on granulocytes of heterozygous B-hIL17RA mice (H/+) but not in WT mice (+/+), and mouse IL17RA was detectable in WT mice (+/+) and heterozygous B-hIL17RA mice (H/+).
