Basic Information
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Targeting strategy
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The exons 2-10 of mouse IL17RB gene that encode the extracellular domain were replaced by human IL17RB exons 2-10 in B-hIL17RB mice.
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mRNA expression analysis
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Strain specific analysis of IL17RB gene expression in wild type (WT) mice and B-hIL17RB mice by RT-PCR.
Mouse Il17rb mRNA was detectable only in thymus of WT mice (+/+). Human IL17RB mRNA was detectable only in homozygous B-hIL17RB mice (H/H) but not in WT mice (+/+).
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Protein expression analysis in CD45+ cells
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Strain specific IL17RB expression analysis in homozygous B-hIL17RB mice by flow cytometry.
Hepatocytes were collected from wild type (WT) mice (+/+) and homozygous B-hIL17RB mice (H/H), and analyzed by flow cytometry with species-specific anti-IL17RB antibody. Mouse IL17RB was detectable in WT mice (+/+) mice due to the cross-reactivity of antibodies. Human IL17RB was detectable in homozygous B-hIL17RB mice (H/H).
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Protein expression analysis in macrophages
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Strain specific IL17RB expression analysis in homozygous B-hIL17RB mice by flow cytometry.
Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hIL17RB mice (H/H), and analyzed by flow cytometry with species-specific anti-IL17RB antibody. Mouse IL17RB was detectable in WT mice (+/+) mice due to the cross-reactivity of antibodies. Human IL17RB was detectable in homozygous B-hIL17RB mice (H/H).
