Basic Information
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Gene Targeting Strategy
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Gene targeting strategy for B-hIL17RB mice. The exons 2-10 of mouse IL17RB gene that encode the extracellular domain were replaced by human IL17RB exons 2-10 in B-hIL17RB mice.
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mRNA Expression Analysis
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Strain specific analysis of IL17RB gene expression in wild type (WT) mice and B-hIL17RB mice by RT-PCR. Mouse Il17rb mRNA was detectable only in thymus of WT mice (+/+). Human IL17RB mRNA was detectable only in homozygous B-hIL17RB mice (H/H) but not in WT mice (+/+).
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Immune Cell Analysis
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Protein expression analysis in CD45+ cells
Strain specific IL17RB expression analysis in homozygous B-hIL17RB mice by flow cytometry. Hepatocytes were collected from wild type (WT) mice (+/+) and homozygous B-hIL17RB mice (H/H), and analyzed by flow cytometry with species-specific anti-IL17RB antibody. Mouse IL17RB was detectable in WT mice (+/+) mice due to the cross-reactivity of antibodies. Human IL17RB was detectable in homozygous B-hIL17RB mice (H/H).
Protein expression analysis in macrophages
Strain specific IL17RB expression analysis in homozygous B-hIL17RB mice by flow cytometry. Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hIL17RB mice (H/H), and analyzed by flow cytometry with species-specific anti-IL17RB antibody. Mouse IL17RB was detectable in WT mice (+/+) mice due to the cross-reactivity of antibodies. Human IL17RB was detectable in homozygous B-hIL17RB mice (H/H). -
Summary
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mRNA expression analysis:
Mouse IL17RB mRNA was detectable in thymocytes of WT mice (+/+). Human IL17RB mRNA was exclusively detectable in homozygous B-hIL17RB mice (H/H) but not in WT mice (+/+).
Protein expression analysis:
Human IL17RB was exclusively detectable on CD45+ cells and macrophages of homozygous B-hIL17RB mice (H/H) but not in WT mice (+/+), and mouse IL17RB was detectable only in WT mice (+/+).
