Basic Information

Strain Name
C57BL/6-Il17rbtm1(IL17RB)/Bcgen
Common name
B-hIL17RB mice
Catalog number
110090
Background
C57BL/6
Aliases
IL17RB, CRL4, EVI27, IL17BR, IL17RH1, interleukin 17 receptor B

Description

IL17RB (interleukin 17 receptor B, gene ID 55540), also known asCRL4 or EVI27 or IL17BR or IL17RH1, encodes a single-pass type I membrane protein. The protein is a cytokine receptor of the interleukin 17 (IL-17) family of cytokines. Human and mouse IIL-17 RB share 76% amino acid (aa) sequence identity. By Northern blot analysis, human IL-17 RB is highly expressed in kidneys and liver and at moderate level in small intestine, colon, and brain, but is expressed at lower levels in testes, brain, small intestine and other endocrine tissues. The expression of IL-17 RB is significantly up-regulated under inflammatory conditions. IL17RB specifically binds strongly to IL-17E and weakly to IL-17B, but does not bind IL-17, IL-17C, and IL-17F. This receptor has been shown to mediate the activation of NF-kappa B and the production of IL8 induced by IL17E. Mice that are homozygous for a null allele have defects in their response to IL17A or IL17F. At present, there are no marketed and clinical drugs, and drugs targeting this gene are all in the early stages of drug development.

Targeting strategy

The exons 2-10 of mouse IL17RB gene that encode the extracellular domain were replaced by human IL17RB exons 2-10 in B-hIL17RB mice.

mRNA expression analysis

Strain specific analysis of IL17RB gene expression in wild type (WT) mice and B-hIL17RB mice by RT-PCR.

Mouse Il17rb mRNA was detectable only in thymus of WT mice (+/+). Human IL17RB mRNA was detectable only in homozygous B-hIL17RB mice (H/H) but not in WT mice (+/+).

Protein expression analysis in CD45+ cells

Strain specific IL17RB expression analysis in homozygous B-hIL17RB mice by flow cytometry.

Hepatocytes were collected from wild type (WT) mice (+/+) and homozygous B-hIL17RB mice (H/H), and analyzed by flow cytometry with species-specific anti-IL17RB antibody. Mouse IL17RB was detectable in WT mice (+/+) mice due to the cross-reactivity of antibodies. Human IL17RB was detectable in homozygous B-hIL17RB mice (H/H).

Protein expression analysis in macrophages

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Strain specific IL17RB expression analysis in homozygous B-hIL17RB mice by flow cytometry.

Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hIL17RB mice (H/H), and analyzed by flow cytometry with species-specific anti-IL17RB antibody. Mouse IL17RB was detectable in WT mice (+/+) mice due to the cross-reactivity of antibodies. Human IL17RB was detectable in homozygous B-hIL17RB mice (H/H).