Basic Information

Strain Name
C57BL/6-Il17rbtm1(IL17RB)/Bcgen
Common name
B-hIL17RB mice
Catalog number
110090
Background
C57BL/6J
NCBI Gene ID
Aliases
IL17RB, CRL4, EVI27, IL17BR, IL17RH1, interleukin 17 receptor B

Targeting strategy

The exons 2-10 of mouse IL17RB gene that encode the extracellular domain were replaced by human IL17RB exons 2-10 in B-hIL17RB mice.

mRNA expression analysis

Strain specific analysis of IL17RB gene expression in wild type (WT) mice and B-hIL17RB mice by RT-PCR.

Mouse Il17rb mRNA was detectable only in thymus of WT mice (+/+). Human IL17RB mRNA was detectable only in homozygous B-hIL17RB mice (H/H) but not in WT mice (+/+).

Protein expression analysis in CD45+ cells

Strain specific IL17RB expression analysis in homozygous B-hIL17RB mice by flow cytometry.

Hepatocytes were collected from wild type (WT) mice (+/+) and homozygous B-hIL17RB mice (H/H), and analyzed by flow cytometry with species-specific anti-IL17RB antibody. Mouse IL17RB was detectable in WT mice (+/+) mice due to the cross-reactivity of antibodies. Human IL17RB was detectable in homozygous B-hIL17RB mice (H/H).

Protein expression analysis in macrophages

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Strain specific IL17RB expression analysis in homozygous B-hIL17RB mice by flow cytometry.

Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hIL17RB mice (H/H), and analyzed by flow cytometry with species-specific anti-IL17RB antibody. Mouse IL17RB was detectable in WT mice (+/+) mice due to the cross-reactivity of antibodies. Human IL17RB was detectable in homozygous B-hIL17RB mice (H/H).