Basic Information

Strain Name
Stock Number
Common Name
B-hIL1RAcP mice
Bcgen (Beijing Biocytogen Co., Ltd)
Related Genes
IL1RAP; IL1R3; C3orf13

Gene targeting strategy

Gene targeting strategy for B-hIL1RACP mice. The chimeric IL1RAP chimeric coding sequence (human signal peptide, human extracellular domain, mouse transmembrane domain, and intracellular domain) was inserted after the 5’UTR of mouse Il1rap gene in B-hIL1RACP mice. The insertion disrupts the endogenous murine Il1rap gene, resulting in the absence of mouse transcripts.

mRNA expression analysis

Strain specific analysis of IL1RAcP gene expression in wild type and B-hIL1RAcP mice by RT-PCR. Mouse IL1RAcP mRNA was detectable in liver of wild type mice (+/+). Human IL1RAcP mRNA was detectable only in B-hIL1RAcP mice (H/H) but not in wild type mice.

Protein expression analysis

Strain specific IL1RAcP expression analysis in homozygous B-hIL1RAcP mice by flow cytometry. Macrophages and monocytes were collected from wild type and homozygous B-hIL1RAcP mice (H/H) and analyzed by flow cytometry with species-specific anti-IL1RAcP antibody. Human IL1RAcP were exclusively detectable in homozygous B-hIL1RAcP but not wild type mice.

Functional verification

Splenocytes were collected from wild mice and homozygous B-hIL1RAcP mice and stimulated with IL1/IL33/IL36 protein. The expression of cytokines such as IL6, TNFa, KC/GRO, IL4, IFNg, IL5, and IL12p70 in splenocyte culture medium was detected after 48 hours. The results showed that murine IL1/IL33/IL36 protein effectively activated splenocytes in wild mice and triggered the secretion of related cytokines. Murine IL1B and murine IL33 proteins could effectively activate splenocytes in B-hIL1RAcP mice and trigger the secretion of related cytokines, while murine IL36 protein could not effectively activate B-hIL1RAcP mice splenocytes.

Back to top