Basic Information

Strain name
C57BL/6-Il2tm1(IL2)Il2ratm1(IL2RA)ll2rbtm2(IL2RB/Bcgen
Common name
B-hIL2/hIL2RA/hIL2RB mice
Catalog number
131076
Background
C57BL/6
Aliases
IL2 also known as TCGF, lymphokine IL2RA also known as CD25, IDDM10, IL2R, IMD41, TCGFR, p55 IL2RB also known as CD122.

Targeting strategy

The mouse Il2 gene that encoding the full coding sequence was replaced by human IL2 full coding sequence in B-hIL2/hIL2RA/hIL2RB mice.

The exons 2-6 of mouse Il2ra gene that encodes the extracellular domain were replaced by human IL2RA exons 2-6 in B-hIL2/hIL2RA/hIL2RB mice.

The exons 2~8 of mouse Cd122 gene that encodes the extracellular domain were replaced by human IL2RB exons 2-8 in B-hIL2/hIL2RA/hIL2RB mice.

Protein expression analysis

Strain specific IL2 expression analysis in homozygous B-hIL2/hIL2RA/ hIL2RB mice by ELISA. Serum was collected from wild-type mice (+/+) and homozygous B-hIL2/hIL2RA/ hIL2RB mice (H/H) stimulated with anti-mCD3 and anti-mCD28 in vitro, and analyzed by ELISA with species-specific IL2 ELISA kit. Mouse IL2 was detectable in wild-type mice. Human IL2 was exclusively detectable in homozygous B-hIL2/hIL2RA/ hIL2RB mice but not in wild-type mice.(ND: Not detectable)

Details

IL2RA protein expression in T cells

Strain specific IL2RA expression analysis in homozygous B-hIL2/hIL2RA/hIL2RB mice (H/H) by flow cytometry. Splenocytes were collected from wild-type mice (+/+) and homozygous B-hIL2/hIL2RA/hIL2RB mice (H/H) stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with anti-IL2RA antibody. Mouse IL2RA was detectable in T cells of wild-type mice. Human IL2RA was exclusively detectable in T cells of homozygous B-hIL2/hIL2RA/hIL2RB (H/H) but not in wild-type mice by the species-specific anti-IL2RA antibody.

 

IL2RA protein expression in CD4+ T cells

Strain specific IL2RA expression analysis in homozygous B-hIL2/hIL2RA/hIL2RB mice (H/H) by flow cytometry. Splenocytes were collected from wild-type mice (+/+) and homozygous B-hIL2/hIL2RA/hIL2RB mice (H/H) stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with anti-IL2RA antibody. Mouse IL2RA was detectable in CD4+ T cells of wild-type mice. Human IL2RA was exclusively detectable in CD4+ T cells of homozygous B-hIL2/hIL2RA/hIL2RB (H/H) but not in wild-type mice by the species-specific anti-IL2RA antibody.

 

IL2RA protein expression in CD8+ T cells

Strain specific IL2RA expression analysis in homozygous B-hIL2/hIL2RA/hIL2RB mice (H/H) by flow cytometry. Splenocytes were collected from wild-type mice (+/+) and homozygous B-hIL2/hIL2RA/hIL2RB mice (H/H) stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with anti-IL2RA antibody. Mouse IL2RA was detectable in CD8+ T cells of wild-type mice. Human IL2RA was exclusively detectable in CD8+ T cells of homozygous B-hIL2/hIL2RA/hIL2RB (H/H) but not in wild-type mice by the species-specific anti-IL2RA antibody.

 

 

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