CD132 is a 64-70 kD type I transmembrane glycoprotein of the Ig superfamily, and is also known as common γ chain (γc) or IL-2 receptor γ subunit. It is expressed broadly on T- and B-lymphocytes, NK cells, monocytes, and granulocytes. CD132 is an essential component of cytokine receptors for IL-2, IL-4, IL-7, IL-9, IL-15, and IL-21. Ligand binding induces tyrosine phosphorylation and initiates signaling through a JAK/STAT pathway. CD132 mutation results in X-linked severe combined immune deficiency (XSCID).
Gene targeting strategy for B-hIL2 mice. The targeted mouse Il2 whole genomic sequence was replaced by human IL2 whole genomic sequence in B-hIL2 mice.
Protein expression analysis
Strain specific IL2 expression analysis in homozygous B-hIL2 mice by ELISA.
Serum were collected from WT and heterozygous B-hIL2 (H/+) mice stimulated with anti-CD3ε and anti-CD28 in vivo, and analyzed by ELISA with species-specific IL2 ELISA kit. Mouse IL2 was detectable in WT and heterozygous B-hIL2 mice. Human IL2 was exclusively detectable in heterozygous B-hIL2 but not WT mice.