Protein expression analysis
Species-specific IL2 expression in B-hIL2 mice by ELISA.
Serum were collected from WT and heterozygous B-hIL2 (H/+) mice stimulated with anti-CD3ε and anti-CD28 in vivo, and analyzed by ELISA with species-specific IL2 ELISA kit. Mouse IL2 was detectable in WT and heterozygous B-hIL2 mice. Human IL2 was exclusively detected in heterozygous B-hIL2 mice.