Basic Information
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Targeting strategy
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Gene targeting strategy for B-hIL20 mice.
The exons 1~5 of mouse Il20 gene that encodes the full-length protein was replaced by human IL20 exons 1~5 in B-hIL20 mice.
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Protein expression analysis
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Strain specific IL20 expression analysis in homozygous B-hIL20 mice by ELISA. Serum was collected from wild-type mice (+/+) and homozygous B-hIL20 mice (H/H), and analyzed by ELISA with IL20 ELISA kit. Mouse IL20 was detectable both in wild-type mice and homozygous B-hIL20 mice. Human IL20 was also detectable both in wild-type mice and homozygous B-hIL20 mice. Because the ELISA kits are cross-recognize both human and mouse IL20.
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mRNA expression analysis
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Strain specific analysis of IL20 mRNA expression in wild-type C57BL/6 mice and B-hIL20 mice by RT-PCR. Genital fat pad RNA were isolated from wildtype C57BL/6 mice (+/+) and homozygous B-hIL20 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with human IL20 primers. Human IL20 mRNA was detected in homozygous B-hIL20 mice but not in wild-type mice.
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Summary
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mRNA expression analysis:
Human IL20 mRNA was detected in homozygous B-hIL20 mice but not in wild-type mice.
Protein expression analysis:
Mouse IL20 was detectable both in wild-type mice and homozygous B-hIL20 mice. Human IL20 was also detectable both in wild-type mice and homozygous B-hIL20 mice. Because the ELISA kits are cross-recognize both human and mouse IL20.
