Basic Information
Description
CD132 is a 64-70 kD type I transmembrane glycoprotein of the Ig superfamily, and is also known as common γ chain (γc) or IL-2 receptor γ subunit. It is expressed broadly on T- and B-lymphocytes, NK cells, monocytes, and granulocytes. CD132 is an essential component of cytokine receptors for IL-2, IL-4, IL-7, IL-9, IL-15, and IL-21. Ligand binding induces tyrosine phosphorylation and initiates signaling through a JAK/STAT pathway. CD132 mutation results in X-linked severe combined immune deficiency (XSCID).
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Target Strategy
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The exons 1~8 of mouse Il2rg gene that encode the full-length protein were replaced by human IL2RG exons 1~8 in B-hIL2RG mice.
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Protein Expression Analysis
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Protein expression analysis
Strain specific IL2RG expression analysis in heterozygous B-hIL2RG mice by flow cytometry.
Splenocytes were collected from WT and hemizygous B-hIL2RG (H/+) mice, and analyzed by flow cytometry with species-specific anti-IL2RG antibody. Mouse IL2RG was detectable in WT and hemizygous B-hIL2RG mice. Human IL2RG was exclusively detectable in hemizygous B-hIL2RG but not WT mice.
Strain specific IL2RG expression analysis in heterozygous B-hIL2RG mice by flow cytometry.
Splenocytes were collected from WT and hemizygous B-hIL2RG (H/+) mice, and analyzed by flow cytometry with species-specific anti-IL2RG antibody. Mouse IL2RG was detectable in WT and hemizygous B-hIL2RG mice. Human IL2RG was exclusively detectable in hemizygous B-hIL2RG but not WT mice.
