Basic Information

Strain Name
C57BL/6-Il2rgtm2(IL2RG)/Bcgen
Common Name
B-hIL2RG mice
Background
C57BL/6
Catalog Number
110087
Related Gene
IL2RG (interleukin-2 receptor subunit gamma)
NCBI Gene ID

Target Strategy

The exons 1~8 of mouse Il2rg gene that encode the full-length protein were replaced by human IL2RG exons 1~8 in B-hIL2RG mice.

Protein Expression Analysis

Protein expression analysis

Strain specific IL2RG expression analysis in heterozygous B-hIL2RG mice by flow cytometry.

Splenocytes were collected from WT and hemizygous B-hIL2RG (H/+) mice, and analyzed by flow cytometry with species-specific anti-IL2RG antibody. Mouse IL2RG was detectable in WT and hemizygous B-hIL2RG mice. Human IL2RG was exclusively detectable in hemizygous B-hIL2RG but not WT mice.

Strain specific IL2RG expression analysis in heterozygous B-hIL2RG mice by flow cytometry.

Splenocytes were collected from WT and hemizygous B-hIL2RG (H/+) mice, and analyzed by flow cytometry with species-specific anti-IL2RG antibody. Mouse IL2RG was detectable in WT and hemizygous B-hIL2RG mice. Human IL2RG was exclusively detectable in hemizygous B-hIL2RG but not WT mice.