Basic Information
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Gene Targeting Strategy
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Gene targeting strategy for B-hIL2RG mice. The exons 1~8 of mouse Il2rg gene that encode the full-length protein were replaced by human IL2RG exons 1~8 in B-hIL2RG mice.
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Protein Expression Analysis
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Strain specific IL2RG expression analysis in heterozygous B-hIL2RG mice by flow cytometry. Splenocytes were collected from WT and hemizygous B-hIL2RG (H/+) mice, and analyzed by flow cytometry with species-specific anti-IL2RG antibody. Mouse IL2RG was detectable in WT and hemizygous B-hIL2RG mice. Human IL2RG was exclusively detectable in hemizygous B-hIL2RG but not WT mice.
Strain specific IL2RG expression analysis in heterozygous B-hIL2RG mice by flow cytometry. Splenocytes were collected from WT and hemizygous B-hIL2RG (H/+) mice, and analyzed by flow cytometry with species-specific anti-IL2RG antibody. Mouse IL2RG was detectable in WT and hemizygous B-hIL2RG mice. Human IL2RG was exclusively detectable in hemizygous B-hIL2RG but not WT mice.
