Basic Information

Strain name
C57BL/6-Il3tm1(IL3)/Bcgen
Common name
B-hIL3 mice
Catalog number
110080
Background
C57BL/6
NCBI Gene ID

Target Strategy

Gene targeting strategy for B-hIL3 mice. The exon1-5 of mouse Il3 gene that encodes the full-length protein was replaced by human IL3 exon1-5 in B-hIL3 mice.

mRNA expression analysis

Strain specific analysis of IL3 gene expression in wild-type C57BL/6 mice and homozygous B-hIL3 mice by RT-PCR. Mouse splenocytes were collected and stimulated with ConA for 48 hrs in vitro. Mouse Il3 mRNA was detectable in wild-type C57BL/6 mice (+/+). Human IL3 mRNA was detectable only in homozygous B-hIL3 mice (H/H) but not in wild-type mice.

Summary

Mouse Il3 mRNA was detectable in wild-type C57BL/6 mice. Human IL3 mRNA was detectable only in homozygous B-hIL3 mice.