B-hIL31/hIL31RA/hOSMR mice

Basic Information

Strain name
C57BL/6-Il31tm1(IL31)Il31ratm1(IL31RA) Osmrtm1(OSMR)/Bcgen
Common Name
B-hIL31/hIL31RA/hOSMR mice
Background
C57BL/6
Catalog Number
130746
Aliases
IL31 IL31RA (CRL; GPL; CRL3; GLMR; GLM-R; PLCA2; hGLM-R; IL-31RA; PRO21384; zcytoR17) OSMR (OSMRB; PLCA1; IL-31RB; OSMRbeta; IL-31R-beta)

Targeting strategy

Gene targeting strategy for B-hIL31/hIL31RA/hOSMR mice.

The exons 1-3 of mouse Il31 gene that encode the mature protein were replaced by human IL31 exons 1-3 in B-hIL31/hIL31RA/hOSMR mice.

The chimeric IL31RA CDS was inserted in exon 4 of mouse Il31ra gene in B-hIL31/hIL31RA/hOSMR mice.

The coding sequences of human OSMR extracellular region and mouse Osmr intracellular region were inserted into mouse Osmr gene in B-hIL31/hIL31RA/hOSMR mice.

mRNA expression analysis (IL31/IL31RA)

Strain specific analysis of IL31 and IL31RA gene expression in wild-type mice and homozygous B-hIL31/hIL31RA mice by RT-PCR. Testis was collect from wild type C57BL/6 mice (+/+) and B-hIL31/hIL31RA mice (H/H). Mouse Il31 and Il31ra mRNA were exclusively detectable in wild-type mice. Human IL31 and IL31RA mRNA were exclusively detectable in homozygous B-hIL31/hIL31RA mice, but not in wild type mice.

mRNA and protein expression analysis (OSMR)

Strain specific analysis of OSMR gene expression in wild-type mice and B-hOSMR mice by RT-PCR and western blot. (A) mRNA expression. Mouse Osmr mRNA was exclusively detectable in the kidney of wild-type mice (+/+). Human OSMR mRNA was exclusively detectable in homozygous B-hOSMR mice (H/H). (B) Protein expression. Kidney was collected from wild-type C57BL/6 mice (+/+) and homozygous B-hOSMR mice (H/H), and analyzed by western blot with anti-OSMR antibody. The OSMR protein can be detected in homozygous B-hOSMR mice.

hIL31 induces pruritus in B-hIL31/hIL31RA/hOSMR mice

hIL31 induces pruritus in B-hIL31/hIL31RA/hOSMR mice. hIL-31 are administered to homozygous B-hIL31/hIL31RA/hOSMR mice (female, n=3) on Day0, Day3, Day7, Day10 and record scratching counts for 3h after hIL-31 injection. Record scratching counts before hIL-31 injection as baseline. As shown in the panel, hIL-31 induced pruritus successfully in B-hIL31/hIL31RA/hOSMR mice. An antibody targeting IL-31 signaling  was used as positive control, which had shown a significant antipruritic effect in our model (Validated by client).