Basic Information

Strain name
C57BL/6-Il36atm1(IL36A)/Bcgen
Common Name
B-hIL36A mice
Background
C57BL/6
Catalog number
110092
NCBI Gene ID
Aliases
IL36A, FIL1, FIL1(EPSILON), FIL1E, IL-1F6, IL1(EPSILON), IL1F6, interleukin 36 alpha

Targeting strategy

Gene targeting strategy for B-hIL36A mice.

The exon 2-5 of mouse Il36a gene that encodes the full-length protein were replaced by human IL36A exon 1-4 in B-hIL36A mice.

mRNA expression analysis

Strain specific analysis of IL36A gene expression in wild type (WT) mice and B-hIL36A mice by RT-PCR.

Mouse Il36a mRNA was detectable only in stomach of WT mice (+/+). Human IL36A mRNA was detectable only in homozygous B-hIL36A mice (H/H) but not in WT mice (+/+).

Protein expression analysis

 

Strain specific IL36A expression analysis in homozygous B-hIL36A mice by western blot .

Skin were collected from wild type (WT) mice (+/+) and homozygous B-hIL36A mice (H/H), and analyzed by western blot with anti-IL36A antibody. Mouse IL36A was detectable in WT mice (+/+) due to the cross-reactivity of antibodies. Human IL36A was detectable in homozygous B-hIL36A mice (H/H) but not in WT mice (+/+).

Summary

mRNA expression analysis:

Mouse Il36a mRNA was detectable only in stomach of WT mice (+/+). Human IL36A mRNA was detectable only in homozygous B-hIL36A mice (H/H) but not in WT mice (+/+).

Protein expression analysis:

Human IL36A was detectable in skin of homozygous B-hIL36A mice (H/H) but not in WT mice (+/+), and mouse IL36A was detectable in WT mice (+/+) due to the cross-reactivity of antibodies.