Basic Information

Strain name
Common Name
B-hIL36A mice
Catalog number
IL36A, FIL1, FIL1(EPSILON), FIL1E, IL-1F6, IL1(EPSILON), IL1F6, interleukin 36 alpha

Targeting strategy

Gene targeting strategy for B-hIL36A mice.

The exon 2-5 of mouse Il36a gene that encodes the full-length protein were replaced by human IL36A exon 1-4 in B-hIL36A mice.

mRNA expression analysis

Strain specific analysis of IL36A gene expression in wild type (WT) mice and B-hIL36A mice by RT-PCR.

Mouse Il36a mRNA was detectable only in stomach of WT mice (+/+). Human IL36A mRNA was detectable only in homozygous B-hIL36A mice (H/H) but not in WT mice (+/+).

Protein expression analysis

Strain specific IL36A expression analysis in homozygous B-hIL36A mice by western blot .

Skin were collected from wild type (WT) mice (+/+) and homozygous B-hIL36A mice (H/H), and analyzed by western blot with anti-IL36A antibody. Mouse IL36A was detectable in WT mice (+/+) due to the cross-reactivity of antibodies. Human IL36A was detectable in homozygous B-hIL36A mice (H/H) but not in WT mice (+/+).

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