Basic Information
-
Targeting strategy
-
Gene targeting strategy for B-hIL36R/hIL1RACP mice.
The chimeric IL1RL2 CDS was inserted after the 5’UTR of mouse Il1rl2 gene in B-hIL36R/hIL1RACP mice. The insertion disrupts the endogenous murine Il1rl2 gene, resulting in the absence of mouse transcripts.
The chimeric IL1RAP CDS was inserted after the 5’UTR of mouse Il1rap gene in B-hIL36R/hIL1RACP mice. The insertion disrupts the endogenous murine Il1rap gene, resulting in the absence of mouse transcripts.
-
mRNA expression analysis
-
Strain specific analysis of IL36R gene expression in wild type (WT) mice, B-hIL36R mice and B-hIL36R/hIL1RACP mice by RT-PCR.
Human IL36R mRNA was detectable only in lung of homozygous B-hIL36R mice (H/H) and homozygous B-hIL36R/hIL1RACP mice (H/H;H/H) but not in WT mice (+/+). The positive band was confirmed to be correct by sequencing.
-
Protein expression analysis
-
Strain specific IL1RACP expression analysis in homozygous B-hIL36R/hIL1RACP mice by flow cytometry.
Monocytes, macrophages and neutrophils were collected from wild type (WT) mice (+/+) and homozygous B-hIL36R/hIL1RACP mice (H/H;H/H), and analyzed by flow cytometry with species-specific anti-hIL1RACP antibody. Human IL1RACP was exclusively detectable in homozygous B-hIL36R/hIL1RACP mice (H/H;H/H) but not in WT mice (+/+).
-
Summary
-
mRNA expression analysis:
Human IL36R mRNA was detectable only in lung of homozygous B-hIL36R mice (H/H) and homozygous B-hIL36R/hIL1RACP mice (H/H;H/H) but not in WT mice (+/+). The positive band was confirmed to be correct by sequencing.
Protein expression analysis:
Human IL1RACP was exclusively detectable on monocytes, macrophages and neutrophils of homozygous B-hIL36R/hIL1RACP mice (H/H;H/H) but not in WT mice (+/+).
