Basic Information

Strain name
C57BL/6-Il4tm2(IL4)Il4ratm1(IL4RA) Il13tm1(IL13)//Bcgen
Common name
B-hIL4/hIL4RA/hIL13 mice
Catalog number
131037
Background
C57BL/6N
Aliases
IL4: interleukin 4; IL4RA: interleukin 4 receptor, IL4R; Il13:interleukin 13

Targeting strategy

The exons 1-4 of mouse Il4 gene that encode the full length coding sequence were replaced by human IL4 exons 1-4 in B-hIL4/hIL4RA/hIL13 mice. The exons 4-7 of mouse Il4ra gene that encode the extracellular region coding sequences were replaced by human IL4RA exons 4-7 in B-hIL4/hIL4RA/hIL13 mice. The exons 1-4 of mouse Il13 gene that encode the full length coding sequence were replaced by human IL13 exons 1-4 in B-hIL4/hIL4RA/hIL13 mice.

mRNA expression analysis

Strain specific analysis of IL4, IL4RA and IL13 gene expression in wild-type and B-hIL4/hIL4RA/hIL13 mice by RT-PCR. Mouse Il4, Il4ra and Il13 mRNA was detectable in thymocytes of wild-type mice (+/+) but not in homozygous B-hIL4/hIL4RA/hIL13 mice (H/H). Human IL4, IL4RA and IL13 mRNA was detectable only in homozygous B-hIL4/hIL4RA/hIL13 mice (H/H) but not in wild-type mice.

Protein expression analysis

Strain specific IL4 expression analysis in homozygous B-hIL4/hIL4RA/hIL13 mice by ELISA.

Serum were collected from wild-type mice (+/+) and homozygous B-hIL4/hIL4RA/hIL13 mice (H/H) stimulated with anti-CD3ε in vivo, and analyzed by ELISA with species-specific IL4 ELISA kit. Mouse IL4 was detectable in wild-type mice. Human IL4 was exclusively detectable in homozygous B-hIL4/hIL4RA/hIL13  mice but not in wild-type mice.

Strain specific IL13 expression analysis in homozygous B-hIL4/hIL4RA/hIL13 mice by ELISA.

CD4+ T cells were collected from wild-type mice (+/+) and homozygous B-hIL4/hIL4RA/hIL13 mice (H/H), and then induction of CD4 + T cells differentiation into Th2 cells, supernatants from Th2 cells culture was analyzed by ELISA with species-specific IL13 ELISA kit. Mouse IL13 was detectable in wild-type mice. Human IL13 was exclusively detectable in homozygous B-hIL4/hIL4RA/hIL13  mice but not in wild-type mice.

Strain specific IL4RA expression analysis in homozygous B-hIL4/hIL4RA/hIL13 mice by flow cytometry. Splenocytes were collected from wild-type mice and homozygous B-hIL4/hIL4RA/hIL13 mice (H/H), and analyzed by flow cytometry with species-specific anti-IL4RA antibody. Mouse IL4RA was detectable in wild-type mice. Human IL4RA was exclusively detectable in homozygous B-hIL4/hIL4RA/hIL13 mice but not in wild-type mice.