IL4R (interleukin 4 receptor) This gene encodes the alpha chain of the IL4 receptor and belongs to the type I transmembrane protein, which plays a huge role in the immune response. IL4R can bind to IL4 and IL13 to regulate the production of IgE, and binding to IL4 can promote the differentiation of Th2 cells, thereby inducing the occurrence of asthma.
Antagonists of IL4 and IL4R can reduce the level of IL4 in the body, and can exert anti-inflammatory and anti-immune effects on asthma patients. IL4 and IL4R are considered to be star targets for the treatment of allergies and asthma.
Protein expression analysis
Strain specific IL4RA expression analysis in homozygous B-hIL4RA mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hIL4RA (H/H) mice stimulated with or without anti-CD3ε in vivo, and analyzed by flow cytometry with species-specific anti-IL4RA antibody. Mouse Il4ra was detectable in WT mice. Human IL4RA was exclusively detectable in homozygous B-hIL4RA mice but not WT mice.
Blood routine test in B-hIL4RA mice
Complete blood count (CBC). Blood from female C57BL/6 and B-hIL4RA mice (n=3, 7-week-old) was analyzed with CBC. There was no differences among any measurement between C57BL/6 and B-hIL4RA mice, indicating that introduction of hIL4RA in place of its mouse counterpart does not change blood cell composition. Values are expressed as mean ± SEM.
Blood chemistry of B-hIL4RA mice
Blood chemistry tests of B-hIL4RA mice. Serum from the C57BL/6 and B-hIL4RA mice (n=3, 7-week-old) was analyzed for levels of ALT (alanine aminotransferase) and AST (aspartate aminotransferase). There was no differences on either measurement between C57BL/6 and B-hIL4RA mice, indicating that introduction of hIL4RA in place of its mouse counterpart does not change ALT and AST levels or health of liver. Values are expressed as mean ± SEM.
Antibody binding assay
Analysis of splenocytes of B-hIL4RA mice by FACS. Splenocytes were isolated from female B-hIL4RA mice (n=3, 6 week-old). Flow cytometry analysis of the splenocytes was performed to assess human IL4RA expression on B cells. Single live cells were gated for CD45 population and used for further analysis as indicated here. Human IL4RA expression was detectable on B cells in B-hIL4RA mice as evidenced by dupilumab (in house) and anti-hIL4RA binding vs isotype control.