Basic Information
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Targeting strategy
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Gene targeting strategy for B-hIL9 mice.
The exons 1-5 of mouse Il9 gene that encodes the full-length protein was replaced by human IL9 exons 1-5 in B-hIL9 mice.
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Protein expression analysis
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Strain specific IL9 expression analysis in homozygous B-hIL9 mice by ELISA. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hIL9 mice (H/H). The CD4+ T cells were isolated from splenocytes and then Induced into Th2 cells in vitro. Supernatant of Th2 cell culture was analyzed by ELISA with species-specific IL9 ELISA kit. Mouse IL9 was detectable in wild-type mice. Human IL9 was exclusively detectable in homozygous B-hIL9 mice but not in wild-type mice.
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Summary
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Protein expression analysis:
Human IL9 was exclusively detectable in Th2 cells of homozygous B-hIL9 mice but not in wild-type mice.
