Basic Information

Strain name
C57BL/6-Liftm1(LIF)Bcgen/Bcgen
Common Name
B-hLIF mice
Background
C57BL/6N
Catalog Number
111199
Aliases
CDF, DIA, HILDA, MLPLI
NCBI Gene ID

Gene targeting strategy

Gene targeting strategy for B-hLIF mice. Exons 2-4 of mouse Lif gene that encodes the full-length protein was replaced by human LIF exon 1-3 in B-hLIF mice.

Protein expression analysis

Species-specific LIF protein expression analysis in wild-type and humanized B-hLIF mice. Bone marrow cells were isolated from wild-type C57BL/6 (+/+) and homozygous B-hLIF (H/H) mice, stimulated with LPS in vitro, and analyzed by ELISA using species-specific LIF kits. Mouse LIF was detected in wild-type mice, while human LIF was detected in B-hLIF mice.

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