CDF, DIA, HILDA, MLPLI
NCBI Gene ID
Gene targeting strategy
Gene targeting strategy for B-hLIF mice. Exons 2-4 of mouse Lif gene that encodes the full-length protein was replaced by human LIF exon 1-3 in B-hLIF mice.
Protein expression analysis
Species-specific LIF protein expression analysis in wild-type and humanized B-hLIF mice. Bone marrow cells were isolated from wild-type C57BL/6 (+/+) and homozygous B-hLIF (H/H) mice, stimulated with LPS in vitro, and analyzed by ELISA using species-specific LIF kits. Mouse LIF was detected in wild-type mice, while human LIF was detected in B-hLIF mice.