Basic Information

Strain name
C57BL/6-Ltatm1(LTA)Tnftm1(TNF)Ltbtm1(LTB)/Bcgen
Common name
B-hLTA/hTNFA/hLTB mice
Background strain
C57BL/6
Catalog number
130769
Alias
LTA:LT, TNFB, TNFSF1, TNLG1E; TNF:DIF, TNF-alpha, TNFA, TNFSF2, TNLG1F; LTB:TNFC, TNFSF3, TNLG1C, p33
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hLTA/hTNFA/hLTB mice.

The targeted mouse Lta, Tnfα and Ltb whole genomic sequence was replaced by human LTA, TNFα and LTB whole genomic sequence in B-hLTA/hTNFA/hLTB mice.

mRNA expression analysis

Strain specific analysis of LTA, TNFA, LTB gene expression in homozygous B-hLTA/hTNFA/hLTB mice by RT-PCR. Mouse Lta, Tnfa and Ltb mRNA were detectable in splenocytes of wild-type (+/+) mice. Human LTA, TNFα and LTB mRNA were detectable only in homozygous B-hLTA/hTNFA/hLTB mice (H/H), but not in wild-type mice.

Protein expression analysis

Strain specific LTA expression analysis in homozygous B-hLTA/hTNFA/hLTB mice by ELISA. Serum was collected from wild-type mice (+/+) and homozygous B-hLTA/hTNFA/hLTB mice (H/H) stimulated with LPS in vivo, and analyzed by ELISA with species-specific LTA ELISA kit. Mouse LTA was both detectable in wild-type mice and B-hLTA/hTNFA/hLTB mice, as the antibody is crossly reactive with human LTA.

Strain specific TNFA expression analysis in homozygous B-hLTA/hTNFA/hLTB mice by ELISA. Serum was collected from wild-type mice (+/+) and homozygous B-hLTA/hTNFA/hLTB mice (H/H) stimulated with LPS in vivo, and analyzed by ELISA with species-specific TNFα ELISA kit. Mouse TNFα was detectable in wild-type mice. Human TNFα was detectable in homozygous B-hLTA/hTNFA/hLTB mice.

Strain specific LTB expression analysis in homozygous B-hLTA/hTNFA/hLTB mice by western blot. Spleen and thymus were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hLTA/hTNFA/hLTB mice (H/H), and analyzed by western blot with anti-LTB antibody. Mouse LTB was detectable both in wild-type mice and homozygous B-hLTA/hTNFA/hLTB mice, as the antibody is crossly reactive with human LTB.