Protein expression analysis
Species-specific MIF protein expression analysis in wild-type and humanized B-hMIF mice. Following LPS stimulation in vivo, serum was isolated from wild-type (+/+) and homozygous B-hMIF (H/H) mice, and analyzed using species-specific MIF ELISA kits. Murine MIF protein was detected in wild-type mice, while human MIF protein was exclusively detected in B-hMIF mice. Values are expressed as mean ± SEM. ND: not detectable.