Basic Information

Strain Name
C57BL/6N-Miftm1(MIF)Bcgen/Bcgen
Common Name
B-hMIF mice
Background
C57BL/6N
Catalog number
111047
Related Genes
MIF, GIF, GLIF, MMIF, macrophage migration inhibitory factor
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hMIF mice. 

The exons 1-3 of mouse Mif gene that encode the full-length protein were replaced by human MIF exons 1-3 in B-hMIF mice.

Protein expression analysis

Strain specific MIF expression analysis in wild type (WT) mice and B-hMIF mice by ELISA. Serum were collected from WT mice (+/+) and homozygous B-hMIF mice (H/H) stimulated with LPS in vivo, and analyzed by ELISA with species-specific MIF ELISA kit. Mouse MIF was detectable in WT mice. Human MIF was exclusively detectable in homozygous B-hMIF mice (H/H) but not WT mice (+/+) . Values are expressed as mean ± SEM. ND: not detectable.

Summary

Protein expression analysis:

Human MIF was exclusively detectable in serum of homozygous B-hMIF mice (H/H) but not WT mice (+/+), and mouse MIF was detectable only in WT mice (+/+).

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