Basic Information

Strain Name
C57BL/6-Serpine1tm1(SERPINE1)/Bcgen
Common Name
B-hPAI1 mice
Background
C57BL/6
Catalog Number
111119
Related Gene
SERPINE1 serpin family E member 1; PAI; PAI1; PAI-1; PLANH1
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hPAI1 mice. 

The exon 2 of mouse Pai1 gene that encode the full-length protein was replaced by human PAI1 exon 2 in B-hPAI1 mice.

mRNA expression analysis

Species-specific PAI1 gene expression analysis in wild-type and humanized B-hPAI1 mice by RT-PCR. Murine Pai1 mRNA was detected in wild-type (+/+) mice, while human PAI1 mRNA was exclusively detected in homozygous B-hPAI1 (H/H) mice.

Protein expression analysis

Species-specific PAI1 protein expression analysis in humanized B-hPAI1 mice by ELISA. Serum was collected from wild-type (+/+) and homozygous B-hPAI1 (H/H) mice stimulated by LPS (2 μg/g body weight) for 8h, and analyzed by ELISA. Murine PAI1 protein was detected in wild-type and homozygous B-hPAI1 mice, due to cross-reactivity, while human PAI1 protein was exclusively detected in B-hPAI1 mice. ND: not detected.