Basic Information
-
Targeting strategy
-
Gene targeting strategy for B-hPAI1 mice.
The exon 2 of mouse Pai1 gene that encode the full-length protein was replaced by human PAI1 exon 2 in B-hPAI1 mice.
-
mRNA expression analysis
-
Species-specific PAI1 gene expression analysis in wild-type and humanized B-hPAI1 mice by RT-PCR. Murine Pai1 mRNA was detected in wild-type (+/+) mice, while human PAI1 mRNA was exclusively detected in homozygous B-hPAI1 (H/H) mice.
-
Protein expression analysis
-
Species-specific PAI1 protein expression analysis in humanized B-hPAI1 mice by ELISA. Serum was collected from wild-type (+/+) and homozygous B-hPAI1 (H/H) mice stimulated by LPS (2 μg/g body weight) for 8h, and analyzed by ELISA. Murine PAI1 protein was detected in wild-type and homozygous B-hPAI1 mice, due to cross-reactivity, while human PAI1 protein was exclusively detected in B-hPAI1 mice. ND: not detected.
