Basic Information

Strain Name
C57BL/6-Serpine1tm1(SERPINE1)/Bcgen
Common Name
B-hPAI1 mice
Background
C57BL/6
Catalog Number
111119
Related Gene
SERPINE1 serpin family E member 1; PAI; PAI1; PAI-1; PLANH1
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hPAI1 mice. 

The exon 2 of mouse Pai1 gene that encode the full-length protein was replaced by human PAI1 exon 2 in B-hPAI1 mice.

mRNA expression analysis

Strain specific analysis of m/hPAI1 gene expression in B-hPAI1 mice by RT-PCR.

Mouse Pai1 mRNA was detectable in the wild-type mice (+/+) and Human PAI1 mRNA was detectable only in  B-hPAI1 mice.

Protein expression analysis

Strain specific PAI1 expression analysis in homozygous  B-hPAI1 mice by ELISA.

Serum was collected from wild-type mice (+/+) and homozygous B-hPAI1 mice (H/H) sitimulated by LPS (2 μg/g body weight) for 8h, and analyzed by ELISA. Mouse PAI1 was detectable in wild-type mice and homozygous B-hPAI1 mice as the antibody is crossly reactive with PAI1 in human and mice. Human PAI1 was exclusively detectable in homozygous B-hPAI1 mice. ND: not detected.

Summary

mRNA expression

Mouse PAI1 mRNA was detectable in the wild-type mice (+/+) and Human PAI1 mRNA was detectable only in  B-hPAI1 mice .

Protein expression

Mouse PAI1 was detectable in wild-type mice and homozygous B-hPAI1 mice as the antibody is crossly reactive with PAI1 in human and mice. Human PAI1 was exclusively detectable in homozygous B-hPAI1 mice.