Basic Information
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Gene targeting strategy
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Gene targeting strategy for B-hPPBP/hPF4 mice. The exons 1-3 of mouse Ppbp gene that encode the full-length protein and the exons 1-3 of mouse Pf4 gene that encode the full-length protein including the non-coding region between Ppbp gene and Pf4 gene were replaced by human PPBP exons 1-3 and human PF4 exons 1-3 in B-hPPBP/hPF4 mice.
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Protein expression analysis
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Species-specific PPBP protein expression analysis in wild-type and humanized B-hPPBP/hPF4 mice. Serum was collected from wild-type C57BL/6 (+/+) and homozygous B-hPPBP/hPF4 (H/H) mice and analyzed by ELISA using species-specific anti-PPBP kits. Murine PPBP protein was detected in wild-type mice, while human PPBP protein was detected in B-hPPBP/hPF4 mice.
Species-specific PF4 protein expression analysis in wild-type and humanized B-hPPBP/hPF4 mice. Serum was collected from wild-type C57BL/6 (+/+) and homozygous B-hPPBP/PF4 (H/H) mice and analyzed by ELISA using species-specific anti-PF4 kits. Murine PF4 protein was detected in wild-type mice, while human PF4 protein was detected in B-hPPBP/hPF4 mice.
