Basic Information

Strain Name
C57BL/6-Rspo1tm1(RSPO1)/Bcgen
Common Name
B-hRSPO1 mice
Background
C57BL/6
Catalog Number
110900
Related Gene
R-spondin 1, RSPO; CRISTIN3
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hRSPO1 mice.

The exons 4-6 of mouse Rspo1 gene that encode the full-length protein were replaced by human RSPO1 exons 5-7 in B-hRSPO1 mice.

mRNA expression analysis

Strain specific analysis of RSPO1 gene expression in WT and B-hRSPO1 mice by RT-PCR.

Mouse Rspo1 mRNA was detectable in kidney of wild-type (+/+). Human RSPO1 mRNA was detectable only in H/H but not in +/+ mice.

Protein expression analysis

Strain specific analysis of RSPO1  expression in WT and B-hRSPO1 mice by western blot.

Liver tissue was collected from wild type C57BL/6 mice (+/+) and homozygous B-hRSPO1 mice (H/H), and analyzed by western blot with anti-RSPO1 antibody, which reacts with human and mouse RSPO1 proteins. RSPO1 was detectable in the liver of  B-hRSPO1 mice (H/H) and wild type mice.