Basic Information
Strain Name
C57BL/6-Rspo1tm1(RSPO1)/Bcgen
Common Name
B-hRSPO1 mice
Background
C57BL/6N
Catalog Number
110900
Related Gene
R-spondin 1, RSPO; CRISTIN3
NCBI Gene ID
-
Gene targeting strategy
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Gene targeting strategy for B-hRSPO1 mice. The exons 4-6 of mouse Rspo1 gene that encode the full-length protein were replaced by human RSPO1 exons 5-7 in B-hRSPO1 mice.
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mRNA expression analysis
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Species-specific RSPO1 gene expression analysis in wild-type and humanized B-hRSPO1 mice by RT-PCR. Murine Rspo1 mRNA was detected in kidney tissue isolated from wild-type (+/+) mice, while human RSPO1 mRNA was detected in B-hRSPO1 mice.
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Protein expression analysis
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Species-specific RSPO1 protein expression analysis in wild-type and humanized B-hRSPO1 mice. Liver tissue was isolated from wild-type C57BL/6 (+/+) and homozygous B-hRSPO1 (H/H) mice, and analyzed by western blot using a cross-reactive anti-RSPO1 antibody. RSPO1 protein was detected in wild-type and B-hRSPO1 mice.
