Basic Information

Strain name
C57BL/6-Sort1tm1(SORT1)/Bcgen
Common name
B-hSORT1 mice
Background
C57BL/6
Catalog number
111158
Aliases
Gp95, LDLCQ6, NT3, NTR3
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hSORT1 mice. The coding sequences of human SORT1 was inserted to replace part of murine exon 2. The insertion disrupts the endogenous murine Sort1 gene, resulting in a non-functional transcript in B-hSORT1 mice.

Protein expression analysis

Strain specific SORT1 expression analysis in wild-type C57BL/6 mice and homozygous B-hSORT1 mice by western blot. Brain tissue was collected from wild-type C57BL/6 mice (+/+) and homozygous B-hSORT1 mice (H/H) and analyzed by western blot with anti-SORT1 antibody. Mouse SORT1 was detectable only in wild-type mice. Human SORT1 was detectable only in homozygous B-hSORT1 mice.