Basic Information

Strain name
C57BL/6-Sort1tm1(SORT1)/Bcgen
Common name
B-hSORT1 mice
Background
C57BL/6N
Catalog number
111158
Aliases
Gp95, LDLCQ6, NT3, NTR3
NCBI Gene ID

Gene targeting strategy

Gene targeting strategy for B-hSORT1 mice. The coding sequences of human SORT1 was inserted to replace part of murine exon 2. The insertion disrupts the endogenous murine Sort1 gene, resulting in a non-functional transcript in B-hSORT1 mice.

Protein expression analysis

Species-specific SORT1 protein expression analysis in wild-type and humanized B-hSORT1 mice. Brain tissue was isolated from wild-type (+/+) and homozygous B-hSORT1 (H/H) mice and analyzed by western blot using species-specific anti-SORT1 antibodies. Murine SORT1 protein was detected in wild-type mice, while human SORT1 protein was exclusively detected in B-hSORT1 mice.