Basic Information
Strain Name
C57BL/6-Sosttm1(SOST)/Bcgen
Common Name
B-hSOST mice
Background
C57BL/6
Catalog Number
110111
Related Genes
SOST (Sclerostin), CDD, DAND61, VBCH
NCBI Gene ID
-
Targeting strategy
-
Gene targeting strategy for B-hSOST mice.
The exons 1-2 of mouse Sost gene were replaced by human SOST exons 1-2 in B-hSOST mice.
-
mRNA expression analysis
-
Strain specific analysis of SOST gene expression in C57BL/6(+/+) and homozygous B-hSOST mice by RT-PCR.
Mouse Sost mRNA was detectable only in heart and testis of C57BL/6(+/+) mice. Human SOST mRNA was detectable only in homozygous B-hSOST mice, but not in C57BL/6(+/+) mice.
-
Protein expression analysis
-
Strain specific analysis of SOST gene expression in C57BL/6(+/+) and homozygous B-hSOST(H/H) mice by western blot.
Heart were collected from C57BL/6(+/+) and homozygous B-hSOST (H/H) mice. SOST was detectable in the heart of the C57BL/6(+/+) and B-hSOST (H/H) mice due to the cross-reactivity of antibodies.
