Basic Information

Strain Name
C57BL/6-Sosttm1(SOST)/Bcgen
Common Name
B-hSOST mice
Background
C57BL/6J
Catalog Number
110111
Related Genes
SOST (Sclerostin), CDD, DAND61, VBCH
NCBI Gene ID

Gene targeting strategy

Gene targeting strategy for B-hSOST mice. The exons 1-2 of mouse Sost gene were replaced by human SOST exons 1-2 in B-hSOST mice.

mRNA expression analysis

Species-specific SOST gene expression analysis in wild-type and humanized B-hSOST mice by RT-PCR. Murine Sost mRNA was detected in heart and testis tissues isolated from wild-type C57BL/6 (+/+) mice, while human SOST mRNA was detected in homozygous B-hSOST (H/H) mice.

Protein expression analysis

Species-specific SOST protein expression analysis in wild-type and humanized B-hSOST mice. Heart tissue was isolated from wild-type C57BL/6 (+/+) and homozygous B-hSOST (H/H) mice and analyzed by western blot using a cross-reactive anti-m/hSOST antibody. SOST protein was detected in wild-type and B-hSOST mice.