Basic Information

Strain Name
C57BL/6-Sosttm1(SOST)/Bcgen
Common Name
B-hSOST mice
Background
C57BL/6
Catalog Number
110111
Related Genes
SOST (Sclerostin), CDD, DAND61, VBCH
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hSOST mice.

The exons 1-2 of mouse Sost gene were replaced by human SOST exons 1-2 in B-hSOST mice.

mRNA expression analysis

Strain specific analysis of SOST gene expression in C57BL/6(+/+) and homozygous B-hSOST mice by RT-PCR.

Mouse Sost mRNA was detectable only in heart and testis of C57BL/6(+/+) mice. Human SOST mRNA was detectable only in homozygous B-hSOST mice, but not in C57BL/6(+/+) mice.

 

 

Protein expression analysis

Strain specific analysis of SOST gene expression in C57BL/6(+/+) and homozygous B-hSOST(H/H) mice by western blot.

Heart were collected from C57BL/6(+/+) and homozygous B-hSOST (H/H) mice. SOST was detectable in the heart of the C57BL/6(+/+) and B-hSOST (H/H) mice due to the cross-reactivity of antibodies.