Basic Information

Strain Name
C57BL/6-Tektm1(TEK)/Bcgen
Common Name
B-hTEK mice
Background
C57BL/6
Catalog number
111106
Related Genes
TEK receptor tyrosine kinase, CD202B, GLC3E, TIE-2, TIE2, VMCM, VMCM1
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hTEK mice.

The exons 2-14 of mouse Tek gene were replaced by human TEK exons 2-14 in B-hTEK mice.

mRNA expression analysis

Strain specific analysis of TEK gene expression in wild-type mice and B-hTEK mice by RT-PCR.

Mouse Tek mRNA was detectable in the lung of wild-type mice (+/+). Human TEK mRNA was detectable only in homozygous B-hTEK but not in wild-type mice.

Protein expression analysis

Strain specific TEK expression analysis in homozygous B-hTEK mice by flow cytometry.

Lung was collected from wild-type mice (+/+) and homozygous B-hTEK mice (H/H), and analyzed by flow cytometry with anti-TEK antibody. Mouse TEK was only detectable in wild-type mice. Human TEK was detectable in both homozygous B-hTEK mice and wild-type mice because of the cross-action antibody.

Back to top