Basic Information

Strain name
C57BL/6-Tgm2tm1(TGM2)/Bcgen
Common Name
B-hTGM2 mice
Background
C57BL/6
Catalog number
111028
Aliases
G(h), TG(C), TGC, hTG2, tTG
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hTGM2 mice. The exons 1-13 of mouse Tgm2 gene that encode the full coding sequences were replaced by human TGM2 exons 1-13 in B-hTGM2 mice.

mRNA expression analysis

Strain specific analysis of TGM2 gene expression in wild-type C57BL/6 mice and B-hTGM2 mice by RT-PCR. Mouse Tgm2 mRNA was detectable in ovary of wild-type C57BL/6 mice (+/+) but not in homozygous B-hTGM2 mice (H/H). Human TGM2 mRNA was detectable only in homozygous B-hTGM2 mice but not in wild-type mice.

Protein expression analysis

Strain specific TGM2 expression analysis in homozygous B-hTGM2 mice by flow cytometry. Liver, lung and spleen tissues were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hTGM2 mice (H/H) and analyzed by western blotting with anti-TGM2 antibody. Hela cell line was used as positive control for human TGM2 protein. TGM2 was detectable in wild-type mice and homozygous B-hTGM2 mice with a known cross-reactive anti-TGM2 antibody.

Summary

mRNA expression analysis:

Mouse Tgm2 mRNA was detectable in ovary of wild-type C57BL/6 mice. Human TGM2 mRNA was detectable only in homozygous B-hTGM2 mice.

Protein expression analysis:

Mouse TGM2 was detectable in wild-type mice and homozygous B-hTGM2 mice with a known cross-reactive anti-TGM2 antibody.