Basic Information
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Details
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mRNA expression analysis
Strain specific analysis of TNFA and TNFR2 gene expression in WT and B-hTNFA/TNFR2 mice by RT-PCR. Mouse Tnfα and Tnfr2 mRNA were detectable only in splenocytes of wild-type (+/+) mice. Human TNFA and TNFR2 mRNA were detectable only in H/H, but not in +/+ mice.
Protein expression analysis
Strain specific TNFα expression analysis in homozygous B-hTNFA/hTNFR2 mice by ELISA. Serum were collected from WT and homozygous B-hTNFA/hTNFR2 (H/H) mice stimulated with LPS in vivo, and analyzed by ELISA with species-specific TNFα ELISA kit. Mouse TNFα was detectable in WT mice. Human TNFα was exclusively detectable in homozygous B-hTNFA/hTNFR2 but not WT mice.
Strain specific TNFR2 expression analysis in homozygous B-hTNFA/hTNFR2 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hTNFA/hTNFR2 (H/H) mice, and analyzed by flow cytometry with species-specific anti-TNFR2 antibody. Mouse TNFR2 was detectable in WT mice. Human TNFR2 was exclusively detectable in homozygous B-hTNFA/hTNFR2 but not WT mice.
Strain specific TNFR2 expression analysis in homozygous B-hTNFA/hTNFR2 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hTNFA/hTNFR2 (H/H) mice stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with species-specific anti-TNFR2 antibody. Mouse TNFR2 was detectable in WT mice. Human TNFR2 was exclusively detectable in homozygous B-hTNFA/hTNFR2 but not WT mice.
Strain specific TNFR2 expression analysis in homozygous B-hTNFA/hTNFR2 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hTNFA/hTNFR2 (H/H) mice, and analyzed by flow cytometry with species-specific anti-TNFR2 antibody. Mouse TNFR2 was detectable in WT mice. Human TNFR2 was exclusively detectable in homozygous B-hTNFA/hTNFR2 but not WT mice.
Strain specific TNFR2 expression analysis in homozygous B-hTNFA/hTNFR2 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hTNFA/hTNFR2 (H/H) mice stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with species-specific anti-TNFR2 antibody. Mouse TNFR2 was detectable in WT mice. Human TNFR2 was exclusively detectable in homozygous B-hTNFA/hTNFR2 but not WT mice.
Strain specific TNFR2 expression analysis in homozygous B-hTNFA/hTNFR2 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hTNFA/hTNFR2 (H/H) mice, and analyzed by flow cytometry with species-specific anti-TNFR2 antibody. Mouse TNFR2 was detectable in WT mice. Human TNFR2 was exclusively detectable in homozygous B-hTNFA/hTNFR2 but not WT mice.
Strain specific TNFR2 expression analysis in homozygous B-hTNFA/hTNFR2 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hTNFA/hTNFR2 (H/H) mice stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with species-specific anti-TNFR2 antibody. Mouse TNFR2 was detectable in WT mice. Human TNFR2 was exclusively detectable in homozygous B-hTNFA/hTNFR2 but not WT mice.
