Basic Information

Strain Name
C57BL/6-Tnftm1(TNF)/Bcgen
Common Name
B-hTNFA mice
Background
C57BL/6J
Catalog Number
110002
Aliases
TNF (tumor necrosis factor, TNFA)
NCBI Gene ID

Gene Targeting strategy

Gene targeting strategy for B-hTNFA mice.The targeted mouse Tnf whole genomic sequence was replaced by human TNF whole genomic sequence in B-hTNFA mice.

Protein expression analysis

Species-specific TNFα protein expression analysis in humanized B-hTNFA mice. Serum was collected from wild-type C57BL/6 (+/+) and homozygous B-hTNFA (H/H) mice stimulated with LPS in vivo, and analyzed using species-specific TNFα ELISA kits. Murine TNFα protein was detected in wild-type mice, while human TNFα protein was exclusively detected in B-hTNFA mice.