Basic Information

Strain Name
C57BL/6-Tnftm1(TNF)/Bcgen
Common Name
B-hTNFA mice
Background
C57BL/6
Catalog Number
110002
Aliases
TNF (tumor necrosis factor, TNFA)
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hTNFA mice.The targeted mouse Tnf whole genomic sequence was replaced by human TNF whole genomic sequence in B-hTNFA mice.

Details

Protein expression analysis

Strain specific TNFα expression analysis in homozygous B-hTNFA mice by ELISA.

Serum were collected from WT and homozygous B-hTNFA (H/H) mice stimulated with LPS in vivo, and analyzed by ELISA with species-specific TNFα ELISA kit. Mouse TNFα was detectable in WT mice. Human TNFα was exclusively detectable in homozygous B-hTNFA but not WT mice.