TNF (tumor necrosis factor, TNFA)
NCBI Gene ID
Gene Targeting strategy
Gene targeting strategy for B-hTNFA mice.The targeted mouse Tnf whole genomic sequence was replaced by human TNF whole genomic sequence in B-hTNFA mice.
Protein expression analysis
Species-specific TNFα protein expression analysis in humanized B-hTNFA mice. Serum was collected from wild-type C57BL/6 (+/+) and homozygous B-hTNFA (H/H) mice stimulated with LPS in vivo, and analyzed using species-specific TNFα ELISA kits. Murine TNFα protein was detected in wild-type mice, while human TNFα protein was exclusively detected in B-hTNFA mice.