TNF gene encodes a multifunctional pro-inflammatory cytokine that belongs to the tumornecrosis factor (TNF) superfamily. The tumor necrosis factor (TNF) superfamily is a protein superfamily of type II transmembrane proteins containing TNF homology domain and forming trimers. Members of this superfamily can be released from the cell membrane by extracellular proteolytic cleavage and function as a cytokine. These proteins are expressed predominantly by immune cells and they regulate diverse cell functions, including immune response and inflammation, but also proliferation, differentiation, apoptosis and embryogenesis. TNFα Regulation of immune cells, induction of fever, cachexia, inflammation and apoptosis, inhibition of tumorigenesis and viral replication and response to sepsis.
Gene targeting strategy for B-hTNFA mice.The targeted mouse Tnf whole genomic sequence was replaced by human TNF whole genomic sequence in B-hTNFA mice.
Protein expression analysis
Strain specific TNFα expression analysis in homozygous B-hTNFA mice by ELISA.
Serum were collected from WT and homozygous B-hTNFA (H/H) mice stimulated with LPS in vivo, and analyzed by ELISA with species-specific TNFα ELISA kit. Mouse TNFα was detectable in WT mice. Human TNFα was exclusively detectable in homozygous B-hTNFA but not WT mice.