B-hTNFR2/hTNFR1/hLTBR mice

Basic Information

Strain Name
C57BL/6-Tnfrsf1btm1(TNFRSF1B)Tnfrsf1atm1(TNFRSF1A) Ltbrtm1(LTBR)/Bcgen
Common Name
B-hTNFR2/hTNFR1/hLTBR mice
Background
C57BL/6
Catalog number
140801
Aliases
TNFRSF1B, CD120b, TBPII, TNF-R-II, TNF-R75, TNFBR, TNFR1B, TNFR2, TNFR80, p75, p75TNFR, TNF receptor superfamily member 1B; TNFRSF1A, CD120a, FPF, TBP1, TNF-R, TNF-R-I, TNF-R55, TNFAR, TNFR1, TNFR55, TNFR60, p55, p55-R, p60, TNF receptor superfamily member 1A; LTBR, D12S370, LT-BETA-R, TNF-R-III, TNFCR, TNFR-RP, TNFR2-RP, TNFR3, TNFRSF3, lymphotoxin beta receptor

TNFR1 protein expression analysis in granulocytes

Species-specific TNFR1 protein expression analysis in wild-type and humanized B-hTNFR2/hTNFR1/hLTBR mice. Splenocytes were isolated from wild-type C57BL/6 (+/+) and homozygous B-hTNFR2/hTNFR1/hLTBR (H/H) mice and analyzed by flow cytometry using species-specific anti-TNFR1 antibodies. Mouse TNFR1 protein was detected in wild-type mice, while human TNFR1 protein was detected in B-hTNFR2/hTNFR1/hLTBR mice.

LTBR protein expression analysis in macrophages

Species-specific LTBR protein expression analysis in wild-type and humanized B-hTNFR2/hTNFR1/hLTBR mice. Spleen, blood and bone marrow cells were isolated from wild-type C57BL/6 (+/+) and homozygous B-hTNFR2/hTNFR1/hLTBR (H/H) mice, and analyzed by flow cytometry using species-specific anti-LTBR antibodies. Mouse LTBR protein was detected in wild-type mice, while human LTBR protein was detected in B-hTNFR2/hTNFR1/hLTBR mice.

TNFR2 protein expression analysis in regulatory T cells (Tregs)

Species-specific TNFR2 protein expression analysis in wild-type and humanized B-hTNFR2/hTNFR1/hLTBR mice. Following anti-CD3ε stimulation in vivo, splenocytes were isolated from wild-type C57BL/6 (+/+) and homozygous B-hTNFR2/hTNFR1/hLTBR (H/H) mice, and analyzed by flow cytometry using species-specific anti-TNFR2 antibodies. Mouse TNFR2 protein was detected in wild-type mice, while human TNFR2 protein was detected in B-hTNFR2/hTNFR1/hLTBR mice.