Basic Information

Strain name
C57BL/6J-Flt1tm1(FLT1)Bcgen/Bcgen
Common name
B-hVEGFR1 mice
Background
C57BL/6J
Catalog number
110981
Aliases
FLT1 (FLT; FLT-1; VEGFR1; VEGFR-1)
NCBI Gene ID

Gene targeting strategy

Gene targeting strategy for B-hVEGFR1 mice. The mouse VEGFR1 gene that encode the extracellular domain were replaced by human VEGFR1 counterpart gene in B-hVEGFR1 mice.

mRNA expression analysis

Strain specific analysis of VEGFR1 gene expression in wild-type mice and B-hVEGFR1 mice by RT-PCR. Mouse VEGFR1 mRNA was detectable in lung of wild-type mice (+/+). Human VEGFR1 mRNA was detectable only in homozygous B-hVEGFR1 mice (H/H) but not in wild-type mice.

Protein expression analysis

Strain specific VEGFR1 expression analysis in homozygous B-hVEGFR1 mice by flow cytometry. Pulmonary vascular endothelial cells were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hVEGFR1 mice (H/H), and analyzed by flow cytometry with anti-VEGFR1 antibody. Human VEGFR1 was detected in homozygous B-hVEGFR1 mice but not in wild-type mice. Mouse VEGFR1 was detectable in wild type mice and homozygous B-hVEGFR1 mice, as the antibody is cross-reactive with VEGFR1 in human and mice.

Summary

mRNA and Protein expression analysis: Human VEGFR1 mRNA and protein were detectable in B-hVEGFR1 mice.

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