Basic Information
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Gene targeting strategy
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Gene targeting strategy for B-hVWF mice. Part of exon 2 and all of exons 3 of mouse Vwf gene sequences were replaced by human VWF coding sequences that encodes the full-length protein, 3’UTR of mouse Vwf gene and STOP termination sequences in B-hVWF mice. The insertion disrupts the endogenous murine Vwf gene, resulting in a non-functional transcript.
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mRNA expression analysis
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Species-specific VWF gene expression analysis in wild-type and humanized B-hVWF mice by RT-PCR. Murine Vwf mRNA was detected in lung tissue isolated from wild-type C57BL/6 (+/+) mice, while human VWF mRNA was detected in homozygous B-hVWF (H/H) mice.
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Protein expression analysis
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Species-specific VWF protein expression analysis in wild-type and humanized B-hVWF mice. Plasma was isolated from wild-type C57BL/6 (+/+) and homozygous B-hVWF (H/H) mice and analyzed by ELISA using species-specific VWF kits. Murine VWF protein was detected in wild-type and B-hVWF mice due to the cross-reactive antibody, while human VWF protein was only detected in B-hVWF mice. Values are expressed as mean ± SEM. ND: not detectable.
