Basic Information
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Gene targeting strategy
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Gene targeting strategy for B-Tg(hIL8) mice. The BAC containing the whole human IL8 genome sequence was randomly inserted into mouse genome in B-Tg(hIL8) mice.
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Protein expression analysis (F1)
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Species-specific IL8 expression analysis in B-Tg(hIL8) mice by ELISA. Serum were collected from wild-type C57BL/6 mice and B-Tg(hIL8) mice after stimulated with LPS in vivo, and analyzed by ELISA with species-specific IL8 ELISA kit. Human IL8 was exclusively detectable in B-Tg(hIL8) mice.
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Protein expression analysis (F2)
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Strain specific IL8 expression analysis in B-Tg(hIL8) mice by ELISA. Serum were collected from wild-type C57BL/6 mice and B-Tg(hIL8) mice after stimulated with LPS in vivo, and analyzed by ELISA with species-specific IL8 ELISA kit. Human IL8 was exclusively detectable in B-Tg(hIL8) mice.
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Protein expression analysis (F3)
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Species-specific IL8 expression analysis in B-Tg(hIL8) mice by ELISA. Serum were collected from wild-type C57BL/6 mice and B-Tg(hIL8) mice after stimulated with LPS in vivo, and analyzed by ELISA with species-specific IL8 ELISA kit. Human IL8 was exclusively detectable in B-Tg(hIL8) mice.