Basic Information
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Protein expression analysis
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Protein expression analysis (spleen)
Species-specific C5AR1 protein expression analysis in wild-type and humanized B-hC5AR1 mice. Splenocytes were isolated from wild-type C57BL/6 (+/+) and homozygous B-hC5AR1 (H/H) mice, and analyzed by flow cytometry using species-specific anti-C5AR1 antibodies. Murine C5AR1 protein was detected in wild-type mice, while human C5AR1 protein was exclusively detected in B-hC5AR1 mice.
Protein expression analysis (bone marrow)
Species-specific C5AR1 protein expression analysis in wild-type and humanized B-hC5AR1 mice. Bone marrow was isolated from wild-type C57BL/6 (+/+) and homozygous B-hC5AR1 (H/H) mice, and analyzed by flow cytometry using species-specific anti-C5AR1 antibodies. Murine C5AR1 protein was detected in wild-type mice, while human C5AR1 protein was detected in B-hC5AR1 mice.