Basic Information
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Targeting strategy
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The exon 2 of mouse CB1 gene that encodes the full-length protein was replaced by human CB1 exon 2 in B-hCB1 mice.
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mRNA expression analysis
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Strain specific analysis of CB1 gene expression in wild-type C57BL/6 mice and B-hCB1 mice by RT-PCR. Mouse CB1 mRNA was detectable only in cerebellum and brain cells of wild-type C57BL/6 mice (+/+). Human CB1 mRNA was detectable only in cerebellum and brain cells of homozygous B-hCB1 mice (H/H), but not in wild-type mice.
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Protein expression analysis
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Strain specific CB1 expression analysis in homozygous B-hCB1 mice by western blot. Cerebellum and brain tissue was collected from wild-type C57BL/6 mice (+/+) and homozygous B-hCB1 mice (H/H) and analyzed by western blot with anti-CB1 antibody which was cross reactive between mouse and human. Mouse and human CB1 were detectable in wild-type mice and homozygous B-hCB1 mice.
