Basic Information

Strain name
C57BL/6-Cnr1tm1(CNR1)/Bcgen
Common name
B-hCB1 mice
Catalog number
110943
Background
C57BL/6
Aliases
CANN6, CB-R, CB1, CB1A, CB1K5, CB1R, CNR

Description

CB1 gene encodes one of two cannabinoid receptors. The cannabinoids, principally delta-9-tetrahydrocannabinol and synthetic analogs, are psychoactive ingredients of marijuana. The cannabinoid receptors are members of the guanine-nucleotide-binding protein (G-protein) coupled receptor family, which inhibit adenylate cyclase activity in a dose-dependent, stereoselective and pertussis toxin-sensitive manner. The two receptors have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana.

Targeting strategy

The exon 2 of mouse CB1 gene that encodes the full-length protein was replaced by human CB1 exon 2 in B-hCB1 mice.

mRNA expression analysis

Strain specific analysis of CB1 gene expression in wild-type C57BL/6 mice and B-hCB1 mice by RT-PCR. Mouse CB1 mRNA was detectable only in cerebellum and brain cells of wild-type C57BL/6 mice (+/+). Human CB1 mRNA was detectable only in cerebellum and brain cells of homozygous B-hCB1 mice (H/H), but not in wild-type mice.

Protein expression analysis

Strain specific CB1 expression analysis in homozygous B-hCB1 mice by western blot. Cerebellum and brain tissue was collected from wild-type C57BL/6 mice (+/+) and homozygous B-hCB1 mice (H/H) and analyzed by western blot with anti-CB1 antibody which was cross reactive between mouse and human. Mouse and human CB1 were detectable in wild-type mice and homozygous B-hCB1 mice.