Gene targeting strategy for B-hCB2 mice.
Exon 1 of mouse CB2 (encoding the full-length protein) was replaced by human CB2 exon 1 in B-hCB2 mice.
CB2 mRNA Expression
Strain specific analysis of CB2 gene expression in wild-type mice and B-hCB2 mice by RT-PCR. Mouse CB2 mRNA was detectable in splenocytes of wild-type mice (+/+). Human CB2 mRNA was detectable only in homozygous humanized B-hCB2 mice.