Basic Information
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Details
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mRNA expression analysis
Strain specific analysis of CCR4 gene expression in WT and hCCR4 mice by RT-PCR. Mouse Ccr4 mRNA was detectable only in splenocytes of wild-type (+/+) mice. Human CCR4 mRNA was detectable only in H/H, but not in +/+ mice.
Protein expression analysis in CD4 T cells
Strain specific CCR4 expression analysis in homozygous B-hCCR4 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hCCR4 (H/H) mice, and analyzed by flow cytometry with species-specific anti-CCR4 antibody. Mouse CCR4 was detectable in WT mice. Human CCR4 was exclusively detectable in homozygous B-hCCR4 but not WT mice.
Protein expression analysis in Tregs
Strain specific CCR4 expression analysis in homozygous B-hCCR4 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hCCR4 (H/H) mice, and analyzed by flow cytometry with species-specific anti-CCR4 antibody. Mouse CCR4 was detectable in WT mice. Human CCR4 was exclusively detectable in homozygous B-hCCR4 but not WT mice.
Protein expression analysis in Tregs
Strain specific CCR4 expression analysis in homozygous B-hCCR4 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hCCR4 (H/H) mice, and analyzed by flow cytometry with species-specific anti-CCR4 antibody. Mouse CCR4 was detectable in WT mice. Human CCR4 was exclusively detectable in homozygous B-hCCR4 but not WT mice.
B-CAG-hCCR4 MC38 bind anti-human CCR4 antibody
Analysis of B-CAG-hCCR4 MC38 by FACS. Flow cytometry analysis of the B-CAG-hCCR4 MC38 was performed to assess anti-human CCR4 Ab binding. Single live cells were gated for CD45- population and used for further analysis as indicated here. B-CAG-hCCR4 MC38 can bind well to anti-hCCR4 antibody (mogamulizumab, in house) vs isotype control.
T cells in B-hCCR4 mice bind anti-human CCR4 antibody
Analysis of splenocytes of B-hCCR4 mice by FACS. Splenocytes were isolated from female B-hCCR4 mice (6 week-old). Flow cytometry analysis of the splenocytes was performed to assess anti-human CCR4 Ab binding with Splenocytes. Single live cells were gated for CD45+ population and used for further analysis as indicated here. T cells of the spleen in homozygous B-hCCR4 mice can bind well to anti-hCCR4 antibody (mogamulizumab, in house) vs isotype control.
Tregs in B-hCCR4 mice bind anti-human CCR4 antibody
Analysis of splenocytes of B-hCCR4 mice by FACS. Splenocytes were isolated from female B-hCCR4 mice (6 week-old). Flow cytometry analysis of the splenocytes was performed to assess anti-human CCR4 Ab binding with Splenocytes. Single live cells were gated for CD45+ population and used for further analysis as indicated here. Tregs of the spleen in homozygous B-hCCR4 mice can bind well to anti-hCCR4 antibody (mogamulizumab, in house) vs isotype control.
