Basic Information

Strain Name
C57BL/6-Ccr4tm1(CCR4)/Bcgen
Stock Number
110098
Common Name
B-hCCR4 mice
Source/Investigator
Bcgen (Beijing Biocytogen Co., Ltd)
Related Genes
CCR4 (C-C motif chemokine receptor 4), CD194
Species
C57BL/6J
Appearance
Black
Genotypes
Homozygous
NCBI Gene ID

Details

mRNA expression analysis

Strain specific analysis of CCR4 gene expression in WT and hCCR4 mice by RT-PCR. Mouse Ccr4 mRNA was detectable only in splenocytes of wild-type (+/+) mice. Human CCR4 mRNA was detectable only in H/H, but not in +/+ mice.

Protein expression analysis in CD4 T cells

Strain specific CCR4 expression analysis in homozygous B-hCCR4 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hCCR4 (H/H) mice, and analyzed by flow cytometry with species-specific anti-CCR4 antibody. Mouse CCR4 was detectable in WT mice. Human CCR4 was exclusively detectable in homozygous B-hCCR4 but not WT mice.

Protein expression analysis in Tregs

Strain specific CCR4 expression analysis in homozygous B-hCCR4 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hCCR4 (H/H) mice, and analyzed by flow cytometry with species-specific anti-CCR4 antibody. Mouse CCR4 was detectable in WT mice. Human CCR4 was exclusively detectable in homozygous B-hCCR4 but not WT mice.

Protein expression analysis in Tregs

Strain specific CCR4 expression analysis in homozygous B-hCCR4 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hCCR4 (H/H) mice, and analyzed by flow cytometry with species-specific anti-CCR4 antibody. Mouse CCR4 was detectable in WT mice. Human CCR4 was exclusively detectable in homozygous B-hCCR4 but not WT mice.

B-CAG-hCCR4 MC38 bind anti-human CCR4 antibody

Analysis of B-CAG-hCCR4 MC38 by FACS. Flow cytometry analysis of the B-CAG-hCCR4 MC38 was performed to assess anti-human CCR4 Ab binding. Single live cells were gated for CD45- population and used for further analysis as indicated here. B-CAG-hCCR4 MC38 can bind well to anti-hCCR4 antibody (mogamulizumab, in house) vs isotype control.

T cells in B-hCCR4 mice bind anti-human CCR4 antibody

Analysis of splenocytes of B-hCCR4 mice by FACS. Splenocytes were isolated from female B-hCCR4 mice (6 week-old). Flow cytometry analysis of the splenocytes was performed to assess anti-human CCR4 Ab binding with Splenocytes. Single live cells were gated for CD45+ population and used for further analysis as indicated here. T cells of the spleen in homozygous B-hCCR4 mice can bind well to anti-hCCR4 antibody (mogamulizumab, in house) vs isotype control.

Tregs in B-hCCR4 mice bind anti-human CCR4 antibody

Analysis of splenocytes of B-hCCR4 mice by FACS. Splenocytes were isolated from female B-hCCR4 mice (6 week-old). Flow cytometry analysis of the splenocytes was performed to assess anti-human CCR4 Ab binding with Splenocytes. Single live cells were gated for CD45+ population and used for further analysis as indicated here. Tregs of the spleen in homozygous B-hCCR4 mice can bind well to anti-hCCR4 antibody (mogamulizumab, in house) vs isotype control.