Basic Information
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Targeting strategy
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Gene targeting strategy for B-hCCR7 mice. The full coding sequence of mouse Ccr7 gene was replaced by counterpart human CCR7 gene in B-hCCR7 mice.
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mRNA expression analysis
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Strain specific analysis of CCR7 gene expression in wild-type mice and B-hCCR7 mice by RT-PCR. Mouse Ccr7 mRNA was detectable in spleen of wild-type mice (+/+). Human CCR7 mRNA was detectable only in homozygous B-hCCR7 mice (H/H) but not in wild-type C57BL/6 mice.
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Protein expression analysis
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Strain specific CCR7 expression analysis in homozygous B-hCCR7 mice by flow cytometry. Splenocytes were collected from wild-type mice (+/+) and homozygous B-hCCR7 mice (H/H), and analyzed by flow cytometry with species-specific anti-CCR7 antibody. Mouse CCR7 was exclusively detectable in WT mice. Human CCR7 were exclusively detectable in homozygous B-hCCR7 mice but not in wild-type mice.
Strain specific CCR7 expression analysis in homozygous B-hCCR7 mice by flow cytometry. Splenocytes were collected from wild-type mice (+/+) and homozygous B-hCCR7 mice (H/H), and analyzed by flow cytometry with species-specific anti-CCR7 antibody. Mouse CCR7 was exclusively detectable in WT mice. Human CCR7 were exclusively detectable in homozygous B-hCCR7 mice but not in wild-type mice.
